ArticleJournal of virology2002
Intact microtubules support adenovirus and herpes simplex virus infections.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 157 citations in OpenAlex.
- Herpes simplex virus type-1 cVAC formation in neuronal cells is mediated by dynein motor function and glycoprotein retrieval from the plasma membrane.Journal of virology · 2024Article
- CRM1 Promotes Capsid Disassembly and Nuclear Envelope Translocation of Adenovirus Independently of Its Export Function.Journal of virology · 2022Article
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- A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.PLoS pathogens · 2021Article
- The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission.PLoS pathogens · 2020Article
- Interaction of human dynein light chain 1 (DYNLL1) with enterochelin esterase (Saudi journal of biological sciences · 2020Article
- Endosomes and Microtubles are Required for Productive Infection in Aquareovirus.Virologica Sinica · 2020Article
- Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.Nature communications · 2020Article
- Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers.Life (Basel, Switzerland) · 2019Review
- Impact of dynamin 2 on adenovirus nuclear entry.Virology · 2019Article
- Article
- Role of kinesins in directed adenovirus transport and cytoplasmic exploration.PLoS pathogens · 2018Article
- Concepts in Light Microscopy of Viruses.Viruses · 2018Review
- Review
- Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons.PLoS pathogens · 2018Article
- Distinct functions of diaphanous-related formins regulate HIV-1 uncoating and transport.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Disruption of Microtubules Post-Virus Entry Enhances Adeno-Associated Virus Vector Transduction.Human gene therapy · 2016Article
- Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells.The Journal of cell biology · 2015Article
- Prevention of herpes simplex virus induced stromal keratitis by a glycoprotein B-specific monoclonal antibody.PloS one · 2015Article
- Herpes simplex virus internalization into epithelial cells requires Na+/H+ exchangers and p21-activated kinases but neither clathrin- nor caveolin-mediated endocytosis.Journal of virology · 2014Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capsids and the enclosed DNA of adenoviruses, including the species C viruses adenovirus type 2 (Ad2) and Ad5, and herpesviruses, such as herpes simplex virus type 1 (HSV-1), are targeted to the nuclei of epithelial, endothelial, fibroblastic, and neuronal cells. Cytoplasmic transport of fluorophore-tagged Ad2 and immunologically detected HSV-1 capsids required intact microtubules and the microtubule-dependent minus-end-directed motor complex dynein-dynactin. A recent study with epithelial cells suggested that Ad5 was transported to the nucleus and expressed its genes independently of a microtubule network. To clarify the mechanisms by which Ad2 and, as an independent control, HSV-1 were targeted to the nucleus, we treated epithelial cells with nocodazole (NOC) to depolymerize microtubules and measured viral gene expression at different times and multiplicities of infections. Our results indicate that in NOC-treated cells, viral transgene expression was significantly reduced at up to 48 h postinfection (p.i.). A quantitative analysis of subcellular capsid localization indicated that NOC blocked the nuclear targeting of Ad2 and also HSV-1 by more than 90% at up to 7 h p.i. About 10% of the incoming Texas Red-coupled Ad2 (Ad2-TR) was enriched at the nucleus in microtubule-depleted cells at 5 h p.i. This result is consistent with earlier observations that Ad2-TR capsids move randomly in NOC-treated cells at less than 0.1 micro m/s and over distances of less than 5 micro m, characteristic of Brownian motion. We conclude that fluorophore-tagged Ad2 and HSV-1 particles are infectious and that microtubules play a prominent role in efficient nuclear targeting during entry and gene expression of species C Ads and HSV-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.