ArticleJournal of virology2002
Feline immunodeficiency virus OrfA is distinct from other lentivirus transactivators.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- SAMHD1 specifically restricts retroviruses through its RNase activity.Retrovirology · 2015Article
- Evolution of puma lentivirus in bobcats (Lynx rufus) and mountain lions (Puma concolor) in North America.Journal of virology · 2014Article
- Feline immunodeficiency virus envelope glycoproteins antagonize tetherin through a distinctive mechanism that requires virion incorporation.Journal of virology · 2014Article
- Feline immunodeficiency virus latency.Retrovirology · 2013Review
- Accessory genes confer a high replication rate to virulent feline immunodeficiency virus.Journal of virology · 2013Article
- Transcriptional regulation of latent feline immunodeficiency virus in peripheral CD4+ T-lymphocytes.Viruses · 2012Article
- Article
- Review
- OrfA downregulates feline immunodeficiency virus primary receptor CD134 on the host cell surface and is important in viral infection.Journal of virology · 2010Article
- Feline immunodeficiency virus (FIV) as a model for study of lentivirus infections: parallels with HIV.Current HIV research · 2010Review
- RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region.Nucleic acids research · 2008Article
- Molecular mechanisms of FIV infection.Veterinary immunology and immunopathology · 2008Review
- Feline immunodeficiency virus OrfA alters gene expression of splicing factors and proteasome-ubiquitination proteins.Virology · 2008Article
- Evolution of the long terminal repeat and accessory genes of feline immunodeficiency virus genomes from naturally infected cougars.Virology · 2008Article
- Characterization of a highly pathogenic molecular clone of feline immunodeficiency virus clade C.Journal of virology · 2004Article
- Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Feline immunodeficiency virus ORF-Ais required for virus particle formation and virus infectivity.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The feline immunodeficiency virus (FIV) accessory factor, OrfA, facilitates transactivation of transcription directed by elements of the viral long terminal repeat (LTR). In order to map OrfA domains required for this transactivation, we used N- and C-terminal deletion constructs of the protein, expressed in a Gal4-based transactivation system. The results demonstrated that FIV OrfA, unlike other lentiviral transactivators such as visna virus Tat, is unable to transactivate from minimal promoter-based reporters and requires additional elements of the viral LTR. Stable CrFK-based cell lines were prepared that expressed OrfA to readily detectable levels and in which we were able to demonstrate 32-fold transactivation of an LTR-chloramphenicol acetyltransferase construct. Transactivation was heavily dependent on the presence of an ATF site within the viral LTR. Changing the translation initiation codon context substantially increased the level of production of OrfA from a bicistronic message that also encodes Rev. In the presence of a more favorable context sequence, the upstream expression of OrfA increased 21-fold, with only a 0.5-fold drop in downstream Rev expression. This suggests that Rev translation may occur via an internal ribosomal entry site rather than by leaky scanning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.