Trial reportThe Journal of physiology2002
Combined inhibition of nitric oxide and prostaglandins reduces human skeletal muscle blood flow during exercise.
Trial report in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 2 syntheses or guidelines pooled it, 175 citations in OpenAlex.
- The role of cocoa flavanols in modulating peripheral and cerebral microvascular function in healthy individuals and populations at-risk of cardiovascular disease: a systematic review.Nutrition journal · 2025Pooled it
- Pooled it
- Effects of acute selective cyclooxygenase-2 inhibition on skeletal muscle microvascular oxygenation and exercise tolerance.Experimental physiology · 2025Trial
- L-Citrulline Supplementation Improves Arterial Blood Flow and Muscle Oxygenation during Handgrip Exercise in Hypertensive Postmenopausal Women.Nutrients · 2024Trial
- Five Days of Tart Cherry Supplementation Improves Exercise Performance in Normobaric Hypoxia.Nutrients · 2023Trial
- Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects.The Journal of physiology · 2017Trial
- COX Inhibitor Influence on Skeletal Muscle Fiber Size and Metabolic Adaptations to Resistance Exercise in Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2016Trial
- New Zealand blackcurrant extract improves cycling performance and fat oxidation in cyclists.European journal of applied physiology · 2015Trial
- Impact of polyphenol antioxidants on cycling performance and cardiovascular function.Nutrients · 2014Trial
- Roles of nitric oxide and prostaglandins in the hyperemic response to a maximal metabolic stimulus: redundancy prevails.European journal of applied physiology · 2013Trial
- Combined inhibition of nitric oxide and vasodilating prostaglandins abolishes forearm vasodilatation to systemic hypoxia in healthy humans.The Journal of physiology · 2011Trial
- Nitric oxide, but not vasodilating prostaglandins, contributes to the improvement of exercise hyperemia via ascorbic acid in healthy older adults.American journal of physiology. Heart and circulatory physiology · 2010Trial
- Roles of nitric oxide synthase and cyclooxygenase in leg vasodilation and oxygen consumption during prolonged low-intensity exercise in untrained humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Trial
- Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2009Trial
- Ageing reduces nitric-oxide- and prostaglandin-mediated vasodilatation in exercising humans.The Journal of physiology · 2007Trial
- Nitric oxide synthase inhibition with L-NAME reduces maximal oxygen uptake but not gas exchange threshold during incremental cycle exercise in man.The Journal of physiology · 2004Trial
- Blunted sympathetic vasoconstriction in contracting skeletal muscle of healthy humans: is nitric oxide obligatory?The Journal of physiology · 2003Trial
- The impact of age and exercise training on endothelial-mediated mechanisms of vasodilation during passive leg movement in males.American journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- The effect of sex on the isolated and combined α- and β-adrenergic control of blood flow during handgrip in adults at high altitude: An exploratory study.Physiological reports · 2026Article
- Examination of post-exercise microvascular reactivity in healthy adults.European journal of applied physiology · 2026Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vascular endothelium is an important mediator of tissue vasodilatation, yet the role of the specific substances, nitric oxide (NO) and prostaglandins (PG), in mediating the large increases in muscle perfusion during exercise in humans is unclear. Quadriceps microvascular blood flow was quantified by near infrared spectroscopy and indocyanine green in six healthy humans during dynamic knee extension exercise with and without combined pharmacological inhibition of NO synthase (NOS) and PG by L-NAME and indomethacin, respectively. Microdialysis was applied to determine interstitial release of PG. Compared to control, combined blockade resulted in a 5- to 10-fold lower muscle interstitial PG level. During control incremental knee extension exercise, mean blood flow in the quadriceps muscles rose from 10 +/- 0.8 ml (100 ml tissue)(-1) min(-1) at rest to 124 +/- 19, 245 +/- 24, 329 +/- 24 and 312 +/- 25 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively. During inhibition of NOS and PG, blood flow was reduced to 8 +/- 0.5 ml (100 ml tissue)(-1) min(-1) at rest, and 100 +/- 13, 163 +/- 21, 217 +/- 23 and 256 +/- 28 ml (100 ml tissue)(-1) min(-1) at 15, 30, 45 and 60 W, respectively (P < 0.05 vs. control). In conclusion, combined inhibition of NOS and PG reduced muscle blood flow during dynamic exercise in humans. These findings demonstrate an important synergistic role of NO and PG for skeletal muscle vasodilatation and hyperaemia during muscular contraction.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.