ArticleClinical and diagnostic laboratory immunology2002
Serodiagnosis of mice minute virus and mouse parvovirus infections in mice by enzyme-linked immunosorbent assay with baculovirus-expressed recombinant VP2 proteins.
Article in Clinical and diagnostic laboratory immunology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens.Virology journal · 2019Article
- A field strain of minute virus of mice (MVMm) exhibits age- and strain-specific pathogenesis.The Journal of general virology · 2018Article
- Results of Survey Regarding Prevalence of Adventitial Infections in Mice and Rats at Biomedical Research Facilities.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Data mining strategies to improve multiplex microbead immunoassay tolerance in a mouse model of infectious diseases.PloS one · 2015Article
- Maternal antibodies or nonproductive infections confound the need for rederivation.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Simultaneous detection of antibodies to mouse hepatitis virus recombinant structural proteins by a microsphere-based multiplex fluorescence immunoassay.Clinical and vaccine immunology : CVI · 2011Article
- Serologic prevalence of MPV1 in mouse strains in a commercial laboratory mouse colony determined by using VP1 antigen.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
- Strain- and age-associated variation in viral persistence and antibody response to mouse parvovirus 1 in experimentally infected mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
- Experimental infection of mice with hamster parvovirus: evidence for interspecies transmission of mouse parvovirus 3.Comparative medicine · 2010Article
- Development and validation of a recombinant nucleocapsid protein-based ELISA for detection of the antibody to porcine reproductive and respiratory syndrome virus.Journal of microbiology (Seoul, Korea) · 2009Article
- Visualization of the externalized VP2 N termini of infectious human parvovirus B19.Journal of virology · 2008Article
- Minute virus of mice: antibody response, viral shedding, and persistence of viral DNA in multiple strains of mice.Comparative medicine · 2008Article
- Embryo transfer rederivation of C.B-17/Icr-Prkdc(scid) mice experimentally infected with mouse parvovirus 1.Comparative medicine · 2008Article
- Transmission probabilities of mouse parvovirus 1 to sentinel mice chronically exposed to serial dilutions of contaminated bedding.Comparative medicine · 2008Article
- Lurking in the shadows: emerging rodent infectious diseases.ILAR journal · 2008Article
- Minute virus of mice, a parvovirus, in complex with the Fab fragment of a neutralizing monoclonal antibody.Journal of virology · 2007Article
- The recombinant nonstructural polyprotein NS1 of porcine parvovirus (PPV) as diagnostic antigen in ELISA to differentiate infected from vaccinated pigs.Veterinary research communications · 2006Article
- Simultaneous serodetection of 10 highly prevalent mouse infectious pathogens in a single reaction by multiplex analysis.Clinical and diagnostic laboratory immunology · 2005Article
- Diagnostic testing of mouse and rat colonies for infectious agents.Lab animal · 2003Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mice minute virus (MMV) and mouse parvovirus (MPV) type 1 are the two parvoviruses known to naturally infect laboratory mice and are among the most prevalent infectious agents found in contemporary laboratory mouse colonies. Serologic assays are commonly used to diagnose MMV and MPV infections in laboratory mice; however, highly accurate, high-throughput serologic assays for the detection of MMV- and MPV-infected mice are needed. To this end, the major capsid viral protein (VP2) genes of MMV and MPV were cloned and MMV recombinant VP2 (rVP2) and MPV rVP2 proteins were expressed by using a baculovirus system. MMV rVP2 and MPV rVP2 spontaneously formed virus-like particles that were morphologically similar to empty parvovirus capsids. These proteins were used as antigens in enzyme-linked immunosorbent assays (ELISAs) to detect anti-MMV or anti-MPV antibodies in the sera of infected mice. Sera from mice experimentally infected with MMV (n = 43) or MPV (n = 35) and sera from uninfected mice (n = 30) were used to evaluate the ELISAs. The MMV ELISA was 100% sensitive and 100% specific in detecting MMV-infected mice, and the MPV ELISA was 100% sensitive and 98.6% specific in detecting MPV-infected mice. Both assays outperformed a parvovirus ELISA that uses a recombinant nonstructural protein (NS1) of MMV as antigen. The MMV rVP2 and MPV rVP2 proteins provide a ready source of easily produced antigen, and the ELISAs developed provide highly accurate, high-throughput assays for the serodiagnosis of MMV and MPV infections in laboratory mice.
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