ArticleNucleic acids research2002
Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Single Staphylococcus aureus SauUSI restriction enzyme motors fragment methylated DNA.Nature chemical biology · 2026Article
- Systematic analysis of specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases reveals mechanisms of enzyme- and sequence-specific DNA readout.Nucleic acids research · 2025Article
- Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides.Environmental science and pollution research international · 2024Article
- Conversion of the CG specific M.MpeI DNA methyltransferase into an enzyme predominantly methylating CCA and CCC sites.Nucleic acids research · 2024Article
- Diverse DNA modification in marine prokaryotic and viral communities.Nucleic acids research · 2022Article
- Genetic variation regulates the activation and specificity of Restriction-Modification systems in Neisseria gonorrhoeae.Scientific reports · 2019Article
- Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates.Scientific reports · 2018Article
- Sterol and genomic analyses validate the sponge biomarker hypothesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Relaxed specificity of prokaryotic DNA methyltransferases results in DNA site-specific modification of RNA/DNA heteroduplexes.Journal of applied genetics · 2015Article
- Molecular characterization of a novel temperate sinorhizobium bacteriophage, ФLM21, encoding DNA methyltransferase with CcrM-like specificity.Journal of virology · 2014Article
- Environmentally selected aphid variants in clonality context display differential patterns of methylation in the genome.PloS one · 2014Article
- Diverse functions of restriction-modification systems in addition to cellular defense.Microbiology and molecular biology reviews : MMBR · 2013Review
- Fidelity index determination of DNA methyltransferases.PloS one · 2013Article
- Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage.Nucleic acids research · 2012Article
- Article
- Neisseria gonorrhoeae FA1090 carries genes encoding two classes of Vsr endonucleases.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytosine C5 methyltransferase M.HaeIII recognises and methylates the central cytosine of its canonical site GGCC. Here we report that M.HaeIII can also, with lower efficiency, methylate cytosines located in a wide range of non-canonical sequences. Using bisulphite sequencing we mapped the methyl- cytosine residues in DNA methylated in vitro and in vivo by M.HaeIII. Methyl-cytosine residues were observed in multiple sequence contexts, most commonly, but not exclusively, at star sites (sites differing by a single base from the canonical sequence). The most frequently used star sites had changes at positions 1 and 4, but there is little or no methylation at star sites changed at position 2. The rate of methylation of non-canonical sites can be quite significant: a DNA substrate lacking a canonical site was methylated by M.HaeIII in vitro at a rate only an order of magnitude slower than an otherwise identical substrate containing the canonical site. In vivo methylation of non-canonical sites may therefore be significant and may have provided the starting point for the evolution of restriction-modification systems with novel sequence specificities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.