ArticleNucleic acids research2002
Mutations altering the cleavage specificity of a homing endonuclease.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
- State-of-the-art 2023 on gene therapy for phenylketonuria.Journal of inherited metabolic disease · 2024Review
- Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9.Genes · 2023Review
- A multifunctional system for genome editing and large-scale interspecies gene transfer.Nature communications · 2022Article
- Genome Editing and Designer Crops for the Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Current Status of Gene Therapy Research in Polyglutamine Spinocerebellar Ataxias.International journal of molecular sciences · 2021Review
- Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity.BMB reports · 2020Review
- CRISPR/Cas Derivatives as Novel Gene Modulating Tools: Possibilities and In Vivo Applications.International journal of molecular sciences · 2020Review
- Genome editing for horticultural crop improvement.Horticulture research · 2019Review
- Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.Nucleic acids research · 2018Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- The CRISPR tool kit for genome editing and beyond.Nature communications · 2018Review
- Genetic and metabolic engineering in diatoms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.Nucleic acids research · 2017Article
- Integration of a CD19 CAR into the TCR Alpha Chain Locus Streamlines Production of Allogeneic Gene-Edited CAR T Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil.BMC plant biology · 2016Article
- Systems Metabolic Engineering of Escherichia coli.EcoSal Plus · 2016Review
- Genome-editing Technologies for Gene and Cell Therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Review
- Mechanisms of gene targeting in higher eukaryotes.Cellular and molecular life sciences : CMLS · 2016Review
- Cas9-Guide RNA Directed Genome Editing in Soybean.Plant physiology · 2015Article
- Molecular scissors and their application in genetically modified farm animals.Transgenic research · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The homing endonuclease I-CreI recognizes and cleaves a particular 22 bp DNA sequence. The crystal structure of I-CreI bound to homing site DNA has previously been determined, leading to a number of predictions about specific protein-DNA contacts. We test these predictions by analyzing a set of endonuclease mutants and a complementary set of homing site mutants. We find evidence that all structurally predicted I-CreI/DNA contacts contribute to DNA recognition and show that these contacts differ greatly in terms of their relative importance. We also describe the isolation of a collection of altered specificity I-CreI derivatives. The in vitro DNA-binding and cleavage properties of two such endonucleases demonstrate that our genetic approach is effective in identifying homing endonucleases that recognize and cleave novel target sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.