ArticleNucleic acids research2002
Functional analysis of iceA1, a CATG-recognizing restriction endonuclease gene in Helicobacter pylori.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Helicobacter pylori iceA, clinical outcomes, and correlation with cagA: a meta-analysis.PloS one · 2012Pooled it
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- Methylome evolution suggests lineage-dependent selection in the gastric pathogen Helicobacter pylori.Communications biology · 2023Article
- Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence FactorAsian Pacific journal of cancer prevention : APJCP · 2017Article
- Determination ofMedical journal of the Islamic Republic of Iran · 2017Article
- Divergence between the Highly Virulent Zoonotic Pathogen Helicobacter heilmannii and Its Closest Relative, the Low-Virulence "Helicobacter ailurogastricus" sp. nov.Infection and immunity · 2016Article
- Transcriptome-wide identification of the genes responding to replanting disease in Rehmannia glutinosa L. roots.Molecular biology reports · 2015Article
- Helicobacter pylori virulence genes in the five largest islands of Indonesia.Gut pathogens · 2015Article
- Helicobacter pylori virulence genes and host genetic polymorphisms as risk factors for peptic ulcer disease.Expert review of gastroenterology & hepatology · 2015Review
- Virulence Factors of Helicobacter pylori: A Review.Clinical medicine insights. Gastroenterology · 2014Review
- The significance of virulence factors in Helicobacter pylori.Journal of digestive diseases · 2013Review
- Diverse functions of restriction-modification systems in addition to cellular defense.Microbiology and molecular biology reviews : MMBR · 2013Review
- Cofactor requirement of HpyAV restriction endonuclease.PloS one · 2010Article
- Pathogenesis of Helicobacter pylori infection.Clinical microbiology reviews · 2006Review
- Helicobacter pylori interstrain restriction-modification diversity prevents genome subversion by chromosomal DNA from competing strains.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
iceA1 in Helicobacter pylori is a homolog of nlaIIIR, which encodes the CATG-specific restriction endonuclease NlaIII in Neisseria lactamica. Analysis of iceA1 sequences from 49 H.pylori strains shows that a full-length NlaIII-like ORF is present in 10 strains, including CH4, but in other strains, including strain 60190, the ORFs are truncated due to a variety of mutations. Our goal was to determine whether iceA1 can encode a NlaIII-like endonuclease. Overexpression in Escherichia coli of iceA1 from CH4, but not from 60190, yielded NlaIII-like activity, indicating that the full-length iceA1 is a functional endonuclease gene. Repair of the iceA1 frameshift mutation in strain 60190 and its expression in E.coli yielded functional NlaIII-like activity. We conclude that iceA1 in CH4 is a functional restriction endonuclease gene, while iceA1 in 60190 is not, due to a frameshift mutation, but that its repair restores its restriction endonuclease activity.
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