ArticleJournal of clinical microbiology2002
Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 67 citations in OpenAlex.
- Recent Advances in the Use of Molecular Methods for the Diagnosis of Bacterial Infections.Pathogens (Basel, Switzerland) · 2022Review
- Free-Living Aquatic Turtles as Sentinels ofFrontiers in veterinary science · 2021Article
- Comparative Genomic Analysis of Third-Generation-Cephalosporin-Resistant Escherichia coli Harboring theAntimicrobial agents and chemotherapy · 2020Article
- DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function.Scientific reports · 2017Article
- rpoS-Regulated core genes involved in the competitive fitness of Salmonella enterica Serovar Kentucky in the intestines of chickens.Applied and environmental microbiology · 2015Article
- Diversity and Persistence of Salmonella enterica Strains in Rural Landscapes in the Southeastern United States.PloS one · 2015Article
- Plasmid profile and pulsed-field gel electrophoresis analysis of Salmonella enterica isolates from humans in Turkey.PloS one · 2014Article
- Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection.The open microbiology journal · 2013Article
- Epidemiology of a Salmonella enterica subsp. enterica serovar Typhimurium strain associated with a songbird outbreak.Applied and environmental microbiology · 2012Article
- Phosphorothioate DNA as an antioxidant in bacteria.Nucleic acids research · 2012Article
- Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates.International journal of microbiology · 2012Article
- Tracing shigatoxigenic Escherichia coli O103, O145, and O174 infections from farm residents to cattle.Journal of clinical microbiology · 2007Article
- DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites.Nucleic acids research · 2007Article
- Comparison of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay to pulsed-field gel electrophoresis to determine the effect of repeated subculture and prolonged storage on RFLP patterns of Shiga toxin-producing Escherichia coli O157:H7.Journal of clinical microbiology · 2006Article
- Pulsed-field gel electrophoresis study of Mycobacterium abscessus isolates previously affected by DNA degradation.Journal of clinical microbiology · 2004Article
- Verocytotoxin-producing Escherichia coli in wild birds and rodents in close proximity to farms.Applied and environmental microbiology · 2004Article
- Development of a PCR-restriction fragment length polymorphism assay for the epidemiological analysis of Shiga toxin-producing Escherichia coli.Journal of clinical microbiology · 2004Article
- Variant Salmonella genomic island 1 antibiotic resistance gene cluster containing a novel 3'-N-aminoglycoside acetyltransferase gene cassette, aac(3)-Id, in Salmonella enterica serovar newport.Antimicrobial agents and chemotherapy · 2004Article
- Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States.Applied and environmental microbiology · 2004Article
- Insertions, deletions, and single-nucleotide polymorphisms at rare restriction enzyme sites enhance discriminatory power of polymorphic amplified typing sequences, a novel strain typing system for Escherichia coli O157:H7.Journal of clinical microbiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.