ArticleJournal of clinical microbiology2002
Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
95 citing papers in PubMed.
- 16S pan-bacterial PCR can accurately identify patients with ventilator-associated pneumonia.Thorax · 2017Trial
- Effects of tongue cleaning on bacterial flora in tongue coating and dental plaque: a crossover study.BMC oral health · 2014Trial
- Prevalence of Pseudomonas aeruginosa in Australian wild birds, native wildlife, livestock and domestic animals.Scientific reports · 2026Article
- Cultivation type, season, and soil nematode interactions affect wheat rhizosphere metabarcoding profiles.Frontiers in plant science · 2026Article
- A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers.Microbial biotechnology · 2025Article
- Skin microbiome analysis of a junctional epidermolysis bullosa patient treated with genetically modified stem cells.Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG · 2025Article
- Rapid and reliable species-level identification from clinical samples using 16 S rRNA gene nanopore sequencing analysis.Scientific reports · 2025Article
- Farming System and Nematodes Affect the Rhizosphere Microbiome of Tropical Banana Plants.Environmental microbiology reports · 2025Article
- Patients in hospital with confirmed bacterial airway infection are significantly more likely to have a respiratory virus co-infection.Journal of medical microbiology · 2025Article
- Combining Gram stain and 16S qPCR improved diagnostic accuracy for suspected pneumonia and could become a new metric in the rapid diagnosis of lower respiratory tract infections.Journal of medical microbiology · 2024Article
- Dupilumab Alters Both the Bacterial and Fungal Skin Microbiomes of Patients with Atopic Dermatitis.Microorganisms · 2024Article
- Fermented Goat's Milk Contributes to the Recovery of Iron Deficiency Anemia via Modulation of the Gut Microbiome.Journal of agricultural and food chemistry · 2023Article
- At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device.Analytical chemistry · 2023Article
- Combining 16S Sequencing and qPCR Quantification RevealsBiomolecules · 2023Article
- Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study.Frontiers in dental medicine · 2023Article
- Optimized Method for Bacterial Nucleic Acid Extraction from Positive Blood Culture Broth for Whole-Genome Sequencing, Resistance Phenotype Prediction, and Downstream Molecular Applications.Journal of clinical microbiology · 2022Article
- Detection of Microorganisms in Clinical Sonicated Orthopedic Devices Using Conventional Culture and qPCR.Revista brasileira de ortopedia · 2022Article
- Investigation of Conditions for Capture of LiveBiosensors · 2022Article
- Validation of a multi-species-specific PCR panel to diagnose patients with suspected postoperative bacterial endophthalmitis.Acta ophthalmologica · 2022Article
- Relationships betweenThe Saudi dental journal · 2022Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We describe a novel adaptation of the TaqMan PCR assay which potentially allows for highly sensitive detection of any eubacterial species with simultaneous species identification. Our system relies on a unique multiprobe design in which a single set of highly conserved sequences encoded by the 16S rRNA gene serves as the primer pair and is used in combination with both an internal highly conserved sequence, the universal probe, and an internal variable region, the species-specific probe. A pre-PCR ultrafiltration step effectively decontaminates or removes background DNA. The TaqMan system described reliabAly detected 14 common bacterial species with a detection limit of 50 fg. Further, highly sensitive and specific pathogen detection was demonstrated with a prototype species-specific probe designed to detect Staphylococcus aureus. This assay has broad potential in the clinical arena for rapid and specific diagnosis of infectious diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.