ArticleJournal of clinical microbiology2002
Rapid-cycle PCR and fluorimetry for detection of mycobacteria.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 82 citations in OpenAlex.
- Development and evaluation of a multiplex PCR for simultaneous detection of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Burkholderia pseudomallei in clinical specimens.PLoS neglected tropical diseases · 2026Article
- Characteristics of a lipase ArEstA with lytic activity against drug-resistant pathogen from a novel myxobacterium,Frontiers in microbiology · 2023Article
- The Predatory MyxobacteriumMicroorganisms · 2021Article
- Real time PCR for the rapid identification and drug susceptibility of Mycobacteria present in Bronchial washings.BMC infectious diseases · 2016Article
- Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting.Memorias do Instituto Oswaldo Cruz · 2016Article
- CSF ADA Determination in Early Diagnosis of Tuberculous Meningitis in HIV-Infected Patients.Scientifica · 2016Article
- Utility of a lateral flow assay for culture confirmation ofMedical journal, Armed Forces India · 2016Article
- Linezolid manifests a rapid and dramatic therapeutic effect for patients with life-threatening tuberculous meningitis.Antimicrobial agents and chemotherapy · 2014Article
- Distribution and respiratory activity of mycobacteria in household water system of healthy volunteers in Japan.PloS one · 2014Article
- Specific and Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Samples by Polymerase Chain Reaction.Interdisciplinary perspectives on infectious diseases · 2012Article
- Usefulness of three-channel multiplex real-time PCR and melting curve analysis for simultaneous detection and identification of the Mycobacterium tuberculosis complex and nontuberculous mycobacteria.Journal of clinical microbiology · 2011Article
- Adenosine Deaminase Levels in CSF of Tuberculous Meningitis Patients.Journal of clinical medicine research · 2010Article
- Detection of Mycobacterium avium ss. Paratuberculosis in Blau Syndrome Tissues.Autoimmune diseases · 2010Article
- Evaluation of molecular tools for detection and drug susceptibility testing of Mycobacterium tuberculosis in stool specimens from patients with pulmonary tuberculosis.Journal of clinical microbiology · 2010Article
- Bayesian receiver operating characteristic estimation of multiple tests for diagnosis of bovine tuberculosis in Chadian cattle.PloS one · 2009Article
- Rapid Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by multiplex, real-time PCR.Journal of clinical microbiology · 2009Article
- LightCycler technology in molecular diagnostics.The Journal of molecular diagnostics : JMD · 2009Article
- Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Cross-priming amplification for rapid detection of Mycobacterium tuberculosis in sputum specimens.Journal of clinical microbiology · 2009Article
- Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.The Journal of molecular diagnostics : JMD · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we used LightCycler PCR amplification and product detection by fluorescence resonance energy transfer probes to identify mycobacteria and differentiate between Mycobacterium tuberculosis complex, Mycobacterium avium, and other nontuberculous mycobacteria. Targeting the 16S rRNA gene, three different probes specific for mycobacteria, M. tuberculosis complex, and M. avium were constructed. As few as five genome copies of target nucleic acid were detected by the probes, illustrating the high sensitivity of the system. All 33 mycobacterial species tested but none of the closely related actinomycetes and other bacteria produced a specific fluorescence signal. A specificity of 100% was also demonstrated for the M. tuberculosis complex-specific probe and the M. avium-specific probe. Within 45 min, the LightCycler method correctly detected mycobacteria and specifically identified M. tuberculosis complex and M. avium without any post-PCR sample manipulation. In view of future clinical studies, we also constructed and tested an internal control which could be used to assure successful amplification and detection of mycobacteria. Monitoring of PCR inhibition will be essential for evaluation of this system for direct detection of mycobacteria in clinical specimens. Finally, we tested our system on sputum seeded with mycobacteria and were able to detect as few as 10 organisms. At present, this system is the fastest available method for identification and differentiation of mycobacteria from culture-positive specimens and offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnostic mycobacterial laboratory.
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