ArticleJournal of clinical microbiology2002
Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff microbiology.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 193 citations in OpenAlex.
- Understanding the dandruff flare-up: A cascade of measurable and perceptible changes to scalp health.International journal of cosmetic science · 2025Trial
- Saccharide isomerate ameliorates cosmetic scalp conditions in a Chinese study population.Journal of cosmetic dermatology · 2023Trial
- Longitudinal study of the scalp microbiome suggests coconut oil to enrich healthy scalp commensals.Scientific reports · 2021Trial
- Dandruff Is a Low-Grade Inflammatory Condition Featuring Hyperproliferative Keratinocytes, Interleukin-17-Producing T Cells, and N-Acyl Ethanolamines.Immunity, inflammation and disease · 2026Article
- Metabolomics in Scalp Seborrheic Dermatitis Evidenced Key Changes in Inflammatory Markers Following a 10-Week Scalp Care Scheme with an Anti-dandruff Shampoo.Dermatology and therapy · 2026Article
- Bidirectional Associations Between Seborrheic Dermatitis and Epithelial Barrier Diseases: A Retrospective Cohort Study.Allergy · 2026Article
- Acetic acid produced bymBio · 2025Article
- Decreased skin colonization withFrontiers in medicine · 2024Article
- Article
- Review
- A Case of Topical Ofloxacin-Induced Otomycosis and Literature Review.Mycopathologia · 2021Review
- Targeted Delivery of Zinc Pyrithione to Skin Epithelia.International journal of molecular sciences · 2021Review
- Comparison of Three Skin Sampling Methods and Two Media for CulturingJournal of fungi (Basel, Switzerland) · 2020Article
- Susceptibilities ofHeliyon · 2020Article
- Overview of the Potential Role ofFrontiers in cellular and infection microbiology · 2020Review
- CutaneousFrontiers in cellular and infection microbiology · 2020Review
- Identification and Evaluation of Inhibitors of Lipase fromInternational journal of molecular sciences · 2019Article
- The diversity and abundance of fungi and bacteria on the healthy and dandruff affected human scalp.PloS one · 2019Article
- Comparison of Healthy and Dandruff Scalp Microbiome Reveals the Role of Commensals in Scalp Health.Frontiers in cellular and infection microbiology · 2018Article
- Bacteria Display Differential Growth and Adhesion Characteristics on Human Hair Shafts.Frontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malassezia fungi have been the suspected cause of dandruff for more than a century. Previously referred to as Pityrosporum ovale, Pityrosporum orbiculare, or Malassezia, these fungi are now known to consist of at least seven Malassezia species. Each species has a specific ecological niche, as well as specific biochemical and genetic characteristics. Malassezia yeasts have fastidious culture conditions and exceedingly different growth rates. Therefore, the results of surveys of Malassezia based on culture methods can be difficult to interpret. We developed a molecular technique, terminal fragment length polymorphism analysis, to more accurately survey the ecology of Malassezia yeasts without bias from culture. This technique involves fluorescent nested PCR of the intergenic transcribed spacer (ITS) ITS I and ITS II region ribosomal gene clusters. All known Malassezia species can be differentiated by unique ITS fragment lengths. We have used this technique to directly analyze scalp samples from subjects enrolled in a demographic scalp health study. Results for subjects assigned composite adherent scalp flaking scores (ASFS) <10 were compared to those for subjects assigned composite ASFS >24. Malassezia restricta and M. globosa were found to be the predominant Malassezia species present in both groups. Importantly, we found no evidence of M. furfur in either group, indicating that M. furfur can be eliminated as the causal organism for dandruff. Both groups also showed the presence of non-Malassezia fungi. This method, particularly when it is used in combination with existing fungal ITS databases, is expected to be useful in the diagnosis of multiple other fungal infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.