ArticleJournal of clinical microbiology2002
Detection and quantification of oral treponemes in subgingival plaque by real-time PCR.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 110 citations in OpenAlex.
- Newly identified pathogens associated with periodontitis: a systematic review.Journal of dental research · 2014Pooled it
- The Effect of Mouthwash with Sambucus williamsii var. coreana Extract on Halitosis: A Randomised, Double-Blind, Placebo-Controlled Study.Oral health & preventive dentistry · 2022Trial
- Supragingival Plaque Microbial Signatures Between Radiation Related Caries and Common Caries.International dental journal · 2025Article
- Microbial Coaggregation in the Oral Cavity: Molecular Interactions and Current Insights.International journal of molecular sciences · 2025Review
- Topology and functional characterization of major outer membrane proteins of Treponema maltophilum and Treponema lecithinolyticum.Molecular oral microbiology · 2025Article
- Increased bacterial load ofFrontiers in oral health · 2025Article
- Early detection of oral bacteria causing gum infections and dental caries in children.Iranian journal of microbiology · 2024Article
- Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.Microorganisms · 2023Review
- Comprehensive Analyses of the Bacterial Population in Non-Healing Claw Lesions of Dairy Cattle.Animals : an open access journal from MDPI · 2022Article
- Oral microbiota populations of adult dogs consuming wet or dry foods.Journal of animal science · 2022Article
- Diversity of Treponema denticola and Other Oral Treponeme Lineages in Subjects with Periodontitis and Gingivitis.Microbiology spectrum · 2021Article
- Article
- Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro.The Journal of veterinary medical science · 2021Article
- Quantification of Bacteria in Mouth-Rinsing Solution for the Diagnosis of Periodontal Disease.Journal of clinical medicine · 2021Article
- Detection of Red complex bacteria,Journal of family medicine and primary care · 2020Article
- Roles of TroA and TroR in Metalloregulated Growth and Gene Expression inJournal of bacteriology · 2020Article
- Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry forJournal of clinical microbiology · 2019Article
- Treponema denticola increases MMP-2 expression and activation in the periodontium via reversible DNA and histone modifications.Cellular microbiology · 2018Article
- Comparative analysis of oral treponemes associated with periodontal health and disease.BMC infectious diseases · 2013Article
- Metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study.The open dentistry journal · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral treponemes have been associated with periodontal diseases. We developed a highly sensitive and specific method to detect and quantify cultivable oral treponemes (Treponema denticola, Treponema vincentii, and Treponema medium) in 50 subgingival plaque samples from 13 healthy subjects as well as 37 patients with periodontal diseases using real-time PCR assays with specific primers and a TaqMan probe for each 16S rRNA sequence. The specificity for each assay was examined by using DNA specimens from various treponemal and other bacterial species. The TaqMan real-time PCR was able to detect from 10(3) to 10(8) cells of the oral treponemes, with correlation coefficients as follows: T. denticola, 0.984; T. vincentii, 0.991; and T. medium, 0.984. The frequencies of occurrence of these three oral treponemes in subgingival plaque samples were as follows: T. denticola, 68.0%; T. vincentii, 36.0%; and T. medium, 48.0%. In addition, the number of T. denticola, T. vincentii, and T. medium cells in plaque samples detected by real-time PCR ranged from 3 to 15,184, 1 to 447, and 1 to 7,301 cells/pg of plaque DNA, respectively. Increased numbers of T. denticola cells were detected in plaque samples from deep periodontal pockets, and T. medium was also detected in deep pockets. On the other hand, T. vincentii was mainly found in shallow pockets. These results suggest that various oral treponemes are associated with the formation of each stage of periodontal disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.