ArticleJournal of clinical microbiology2002
Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 83 citations in OpenAlex.
- Comparative evaluation of various diagnostic techniques for detection ofIranian journal of veterinary research · 2022Article
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Presence of Cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system.Infectious diseases of poverty · 2016Article
- Genotyping of Cryptosporidium Species and Their Clinical Manifestations in Patients with Renal Transplantation and Human Immunodeficiency Virus Infection.Journal of pathogens · 2016Article
- Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Development and Evaluation of Three Real-Time PCR Assays for Genotyping and Source Tracking Cryptosporidium spp. in Water.Applied and environmental microbiology · 2015Article
- Development of an immunomagnetic bead separation-coupled quantitative PCR method for rapid and sensitive detection of Cryptosporidium parvum oocysts in calf feces.Parasitology research · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples.Parasitology research · 2012Article
- Development of a direct DNA extraction protocol for real-time PCR detection of Giardia lamblia from surface water.Ecotoxicology (London, England) · 2009Article
- CP2 gene as a useful viability marker for Cryptosporidium parvum.Parasitology research · 2008Article
- Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis.Current microbiology · 2006Article
- Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.Journal of clinical microbiology · 2006Article
- Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.Applied and environmental microbiology · 2006Article
- Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations.Applied and environmental microbiology · 2006Article
- Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens.Journal of clinical microbiology · 2006Article
- Advances in real-time PCR: application to clinical laboratory diagnostics.Advances in clinical chemistry · 2005Review
- Detection and genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by oligonucleotide microarray.Journal of clinical microbiology · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum beta-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was analyzed. Because C. parvum isolates infecting humans comprise two distinct genotypes, designated type 1 and type 2, real-time PCR methods for discriminating C. parvum genotypes were developed. The first method used the same beta-tubulin amplification primers and two fluorescently labeled antisense oligonucleotide probes spanning a 49-bp polymorphic sequence diagnostic for C. parvum type 1 and type 2. The second genotyping method used SYBR Green I fluorescence and targeted a polymorphic coding region within the GP900/poly(T) gene. Both methods discriminated between type 1 and type 2 C. parvum on the basis of melting curve analysis. To our knowledge, this is the first report describing the application of melting curve analysis for genotyping of C. parvum oocysts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.