ArticleBiophysical journal2002
Calcium-induced alterations in mitochondrial morphology quantified in situ with optical scatter imaging.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Optical diffraction tomography for assessing single cell models in angular light scattering.Biomedical optics express · 2024Article
- Three-dimensional angular scattering simulations inform analysis of scattering from single cells.Journal of biomedical optics · 2023Article
- Matching an immersion medium's refractive index to a cell's cytosol isolates organelle scattering.Biomedical optics express · 2022Article
- The Link between Oxidative Stress, Redox Status, Bioenergetics and Mitochondria in the Pathophysiology of ALS.International journal of molecular sciences · 2021Review
- Mitochondrial Morphofunction in Mammalian Cells.Antioxidants & redox signaling · 2019Review
- Differences in forward angular light scattering distributions between M1 and M2 macrophages.Journal of biomedical optics · 2015Article
- Retinal Pigment Epithelial Cells Mitigate the Effects of Complement Attack by Endocytosis of C5b-9.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Isolation of mitochondria with cubic membrane morphology reveals specific ionic requirements for the preservation of membrane structure.Protoplasma · 2015Article
- Plastid osmotic stress activates cellular stress responses in Arabidopsis.Plant physiology · 2014Article
- Investigation of nuclear nano-morphology marker as a biomarker for cancer risk assessment using a mouse model.Journal of biomedical optics · 2012Article
- Mechanosensitive channels protect plastids from hypoosmotic stress during normal plant growth.Current biology : CB · 2012Article
- Ultrasensitive label-free photothermal imaging, spectral identification, and quantification of cytochrome c in mitochondria, live cells, and solutions.Journal of biophotonics · 2010Article
- Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters.Biomedical optics express · 2010Article
- Microscopic imaging and spectroscopy with scattered light.Annual review of biomedical engineering · 2010Review
- Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants.Physiological genomics · 2010Article
- Light scattering measurements of subcellular structure provide noninvasive early detection of chemotherapy-induced apoptosis.Cancer research · 2009Article
- Mitochondrial swelling measurement in situ by optimized spatial filtering: astrocyte-neuron differences.Biophysical journal · 2008Article
- The C-terminal transmembrane domain of Bcl-xL mediates changes in mitochondrial morphology.Biophysical journal · 2008Article
- Scattering of exciting light by live cells in fluorescence confocal imaging: phototoxic effects and relevance for FRAP studies.Biophysical journal · 2007Article
- Light scattering from collagen fiber networks: micro-optical properties of normal and neoplastic stroma.Biophysical journal · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Optical scatter imaging (OSI), a technique we developed recently, was used to measure the ratio of wide-to-narrow angle scatter (OSIR) within endothelial cells subjected to calcium overload (1.6 mM) after permeabilization by ionomycin. Within a few minutes of calcium overload, the mitochondria, which started as elongated organelles, rounded up into spherically shaped particles. This change in morphology was accompanied by a statistically significant 14% increase in OSIR in the cells' cytoplasm. Mitochondrial rounding and OSIR increase were suppressed by cyclosporin A (25 microM), implying that the observed geometrical and scattering changes were directly attributable to the mitochondrial permeability transition. The angular scattering properties of a long mitochondrion rounding up were approximated by numerical simulations of light scatter from an ellipsoid rounding up into a sphere. The simulations predicted a relative increase in OSIR comparable to that measured experimentally for the case where the shape transition takes place with little or no volume increase. The simulations also suggested that mitochondrial refractive index changes could not account for the OSIR changes observed. Our data show that changes in OSIR correlate with mitochondrial morphology change in situ. OSI provides a new tool for subcellular imaging and complements other microscopy methods, such as fluorescence.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.