ArticleBiophysical journal2002
Heterogeneity and persistence length in human ocular mucins.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 106 citations in OpenAlex.
- Imaging of Mucin Networks with Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Mirror Image Mucins and Thio Mucins with Tunable Biodegradation.Journal of the American Chemical Society · 2023Article
- Synthesis and biomedical applications of mucin mimic materials.Advanced drug delivery reviews · 2022Review
- Mucus-Inspired Dynamic Hydrogels: Synthesis and Future Perspectives.Journal of the American Chemical Society · 2022Review
- Length of mucin-like domains enhances cell-Ebola virus adhesion by increasing binding probability.Biophysical journal · 2021Article
- The MUC5B mucin polymer is dominated by repeating structural motifs and its topology is regulated by calcium and pH.Scientific reports · 2019Article
- Equilibrium Modeling of the Mechanics and Structure of the Cancer Glycocalyx.Biophysical journal · 2019Article
- Engineering mucus to study and influence the microbiome.Nature reviews. Materials · 2019Article
- The Interaction of the Gut Microbiota with the Mucus Barrier in Health and Disease in Human.Microorganisms · 2018Review
- Secondary Structure and Glycosylation of Mucus Glycoproteins by Raman Spectroscopies.Analytical chemistry · 2016Article
- Biopolymeric Mucin and Synthetic Polymer Analogs: Their Structure, Function and Role in Biomedical Applications.Polymers · 2016Review
- Chemically tunable mucin chimeras assembled on living cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier.Scientific reports · 2015Article
- Single molecule study of heterotypic interactions between mucins possessing the Tn cancer antigen.Glycobiology · 2015Article
- A combined small-angle X-ray and neutron scattering study of the structure of purified soluble gastrointestinal mucins.Biopolymers · 2014Article
- Silane surface modification for improved bioadhesion of esophageal stents.Applied surface science · 2014Article
- Mining the "glycocode"--exploring the spatial distribution of glycans in gastrointestinal mucin using force spectroscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Supramolecular dynamics of mucus.Cold Spring Harbor perspectives in medicine · 2012Review
- Comparative biomechanics of thick filaments and thin filaments with functional consequences for muscle contraction.Journal of biomedicine & biotechnology · 2010Review
- Cardiac myosin binding protein-C is essential for thick-filament stability and flexural rigidity.Biophysical journal · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy (AFM) has been used to investigate the heterogeneity and flexibility of human ocular mucins and their subunits. We have paid particular attention, in terms of theory and experiment, to the problem of inducing the polymers to assume equilibrium conformations at a surface. Mucins deposited from a buffer containing Ni(2+) ions adopt extended conformations on mica akin to those observed for DNA under similar conditions. The heterogeneity of the intracellular native mucins is evident from a histogram of contour lengths, reflecting, in part, the diversity of mucin gene products expressed. Reduction of the native mucin with dithiothreitol, thereby breaking the S==S bonds between cysteine residues, causes a marked reduction in polymer length. These results reflect the modes of transport and assembly of newly synthesized mucins in vivo. By modifying the worm-like chain model for applicability to two dimensions, we have confirmed that under the conditions employed mucin adsorbs to mica in an equilibrated conformation. The determined persistence length of the native mucin, 36 nm, is consistent with that of an extended, flexible polymer; such characteristics will influence the properties of the gels formed in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.