ArticleBiophysical journal2002
Comparison of the dynamics of the primary events of bacteriorhodopsin in its trimeric and monomeric states.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Actinorhodopsin: an efficient and robust light-driven proton pump for bionanotechnological applications.Scientific reports · 2025Article
- Retinal-Carotenoid Interactions in a Sodium-Ion-Pumping Rhodopsin: Implications on Oligomerization and Thermal Stability.The journal of physical chemistry. B · 2023Article
- Bidirectional Photochemistry of Antarctic Microbial Rhodopsin: Emerging Trend of Ballistic Photoisomerization from the 13-The journal of physical chemistry letters · 2022Article
- Roles of functional lipids in bacteriorhodopsin photocycle in various delipidated purple membranes.Biophysical journal · 2022Article
- X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin.Scientific reports · 2019Article
- Structural and Functional Studies of a Newly Grouped Haloquadratum walsbyi Bacteriorhodopsin Reveal the Acid-resistant Light-driven Proton Pumping Activity.The Journal of biological chemistry · 2015Article
- Tuning the Photocycle Kinetics of Bacteriorhodopsin in Lipid Nanodiscs.Biophysical journal · 2015Article
- Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins.PloS one · 2013Article
- Near-IR resonance Raman spectroscopy of archaerhodopsin 3: effects of transmembrane potential.The journal of physical chemistry. B · 2012Article
- Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system.Biophysical journal · 2012Article
- Cell-free co-expression of functional membrane proteins and apolipoprotein, forming soluble nanolipoprotein particles.Molecular & cellular proteomics : MCP · 2008Article
- Femtosecond stimulated Raman study of excited-state evolution in bacteriorhodopsin.The journal of physical chemistry. B · 2005Article
- The role of small intraprotein cavities in the catalytic cycle of bacteriorhodopsin.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, femtosecond pump-probe spectroscopy in the visible region of the spectrum has been used to examine the ultrafast dynamics of the retinal excited state in both the native trimeric state and the monomeric state of bacteriorhodopsin (bR). It is found that the excited state lifetime (probed at 490 nm) increases only slightly upon the monomerization of bR. No significant kinetic difference is observed in the recovery process of the bR ground state probed at 570 nm nor in the fluorescent state observed at 850 nm. However, an increase in the relative amplitude of the slow component of bR excited state decay is observed in the monomer, which is due to the increase in the concentration of the 13-cis retinal isomer in the ground state of the light-adapted bR monomer. Our data indicate that when the protein packing around the retinal is changed upon bR monomerization, there is only a subtle change in the retinal potential surface, which is dependent on the charge distribution and the dipoles within the retinal-binding cavity. In addition, our results show that 40% of the excited state bR molecules return to the ground state on three different time scales: one-half-picosecond component during the relaxation of the excited state and the formation of the J intermediate, a 3-ps component as the J changes to the K intermediate where retinal photoisomerization occurs, and a subnanosecond component during the photocycle.
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