ArticleBiophysical journal2002
Rapid transbilayer movement of the fluorescent sterol dehydroergosterol in lipid membranes.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 79 citations in OpenAlex.
- An origin for a eukaryotic lipid transfer protein fold in Asgard archaea.bioRxiv : the preprint server for biology · 2025Article
- A cholesterol switch controls phospholipid scrambling by G protein-coupled receptors.The Journal of biological chemistry · 2024Article
- Reconstitution of ORP-mediated lipid exchange process coupled to PI(4)P metabolism.bioRxiv : the preprint server for biology · 2023Article
- Quantitative Models of Lipid Transfer and Membrane Contact Formation.Contact (Thousand Oaks (Ventura County, Calif.)) · 2022Article
- Pathways and Mechanisms of Cellular Cholesterol Efflux-Insight From Imaging.Frontiers in cell and developmental biology · 2022Review
- Steady state analysis of influx and transbilayer distribution of ergosterol in the yeast plasma membrane.Theoretical biology & medical modelling · 2019Article
- Endoplasmic reticulum-plasma membrane contact sites integrate sterol and phospholipid regulation.PLoS biology · 2018Article
- Structural basis of sterol binding and transport by a yeast StARkin domain.The Journal of biological chemistry · 2018Article
- Molecular basis for sterol transport by StART-like lipid transfer domains.The EMBO journal · 2018Article
- STARD3 mediates endoplasmic reticulum-to-endosome cholesterol transport at membrane contact sites.The EMBO journal · 2017Article
- A comparative study on fluorescent cholesterol analogs as versatile cellular reporters.Journal of lipid research · 2016Article
- Fluorescent Sterols and Cholesteryl Esters as Probes for Intracellular Cholesterol Transport.Lipid insights · 2015Review
- Comparative studies on detergent-assisted apocytochrome b6 reconstitution into liposomal bilayers monitored by Zetasizer instruments.PloS one · 2014Article
- Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers.The Journal of cell biology · 2011Article
- STARD4 abundance regulates sterol transport and sensing.Molecular biology of the cell · 2011Article
- Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM.Traffic (Copenhagen, Denmark) · 2011Article
- Noninvasive neutron scattering measurements reveal slower cholesterol transport in model lipid membranes.Biophysical journal · 2011Article
- Mathematical modeling and validation of the ergosterol pathway in Saccharomyces cerevisiae.PloS one · 2011Article
- Effect of sterol carrier protein-2 gene ablation on HDL-mediated cholesterol efflux from cultured primary mouse hepatocytes.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Fatty acids are rapidly delivered to and extracted from membranes by methyl-beta-cyclodextrin.Journal of lipid research · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study establishes a new assay for measuring the transbilayer movement of dehydroergosterol (DHE) in lipid membranes. The assay is based on the rapid extraction of DHE by methyl-beta-cyclodextrin (M-CD) from liposomes. The concentration of DHE in the liposomal membrane was measured by using fluorescence resonance energy transfer (FRET) from DHE to dansyl-phosphatidylethanolamine, which is not extracted from liposomes by M-CD. The method was applied to small (SUV) and large (LUV) unilamellar vesicles of different compositions and at various temperatures. From the kinetics of FRET changes upon extraction of DHE from membranes, rates of M-CD mediated extraction and flip-flop of DHE could be deduced and were found to be dependent on the physical state of the lipid phase. For egg phosphocholine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine in the liquid-crystalline state, halftimes of extraction and transbilayer movement were <5 s and approximately 20-50 s, respectively, at 10 degrees C. For 1,2-dimyristoyl-sn-glycero-3-phosphocholine-SUV being in the gel state at 10 degrees C, the respective halftimes were 28 s and 5-8 min. Surprisingly, DHE could not be extracted from LUV consisting of 1,2-dimyristoyl-sn-glycero-3-phosphocholine. This might be an indication of specific interactions between DHE molecules in membranes depending on the phospholipid composition of the membrane.
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