ArticleApplied and environmental microbiology2002
The fibronectin type 3-like repeat from the Clostridium thermocellum cellobiohydrolase CbhA promotes hydrolysis of cellulose by modifying its surface.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed, 175 citations in OpenAlex.
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- Role of the CBM11, Fn3, and CBM3 Domains in Enhancing the Multifunctional Enzymatic Activities of Glycoside Hydrolase Family 5 fromJournal of microbiology and biotechnology · 2025Article
- The physiological functions of the Cbp2D and Cbp2E proteins are important for insoluble cellulose-dependent growth inApplied and environmental microbiology · 2025Article
- Functional characterization of Lytic Polysaccharide Monooxygenase from Enterococcus mundtii CRi_37 for enhanced stubble saccharification.Molecular biology reports · 2025Article
- Non-Catalytic Domains of Glycoside Hydrolase Family 5 fromJournal of microbiology and biotechnology · 2025Article
- Genomic analysis of a halophilic bacterium Nesterenkonia sp. CL21 with ability to produce a diverse group of lignocellulolytic enzymes.Folia microbiologica · 2025Article
- Flavivirus NS4B proteins do not form homodimers: discrepancies with an AlphaFold-based oligomeric model.Computational and structural biotechnology journal · 2025Article
- Lichenase and Cellobiohydrolase Activities of a Novel Bi-Functional β-Glucanase from the Marine BacteriumMarine drugs · 2024Article
- Unique Fn3-like biosensor in σProtein science : a publication of the Protein Society · 2024Article
- Deciphering domain structures of Aspergillus and Streptomyces GH3-β-Glucosidases: a screening system for enzyme engineering and biotechnological applications.BMC research notes · 2024Article
- Oat Brewery Waste Decreased Methane Production and Alters Rumen Fermentation, Microbiota Composition, and CAZymes Profiles.Microorganisms · 2024Article
- Use of xylosidase 3C from Segatella baroniae to discriminate xylan non-reducing terminus substitution characteristics.BMC research notes · 2024Article
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- Comparative Rumen Metagenome and CAZyme Profiles in Cattle and Buffaloes: Implications for Methane Yield and Rumen Fermentation on a Common Diet.Microorganisms · 2023Article
- The exceptional form and function of the giant bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Current challenges in designer cellulosome engineering.Applied microbiology and biotechnology · 2023Review
- Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366.Scientific reports · 2023Article
- Study of the binding of ΔFN3.1 fragments of theMicrobiome research reports · 2023Article
14 more citing papers are in PubMed but not listed here.
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6 authors at 2 institutions in 1 country.
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Abstract
Fibronectin type 3 homology domains (Fn3) as found in the cellobiohydrolase CbhA of Clostridium thermocellum are common among bacterial extracellular glycohydrolases. The function of these domains is not clear. CbhA is modular and composed of an N-terminal family IV carbohydrate-binding domain (CBDIV), an immunoglobulin-like domain, a family 9 glycosyl hydrolase catalytic domain (Gh9), two Fn3-like domains (Fn3(1,2)), a family III carbohydrate-binding domain (CBDIII), and a dockerin domain. Efficiency of cellulose hydrolysis by truncated forms of CbhA increased in the following order: Gh9 (lowest efficiency), Gh9-Fn3(1,2) (more efficient), and Gh9-Fn3(1,2)-CBDIII (greatest efficiency). Thermostability of the above constructs decreased in the following order: Gh9 (most stable), Gh9-Fn3(1,2), and then Gh9-Fn3(1,2)-CBDIII (least stable). Mixing of Orpinomyces endoglucanase CelE with Fn3(1,2,) or Fn3(1,2)-CBDIII increased efficiency of hydrolysis of acid-swollen cellulose (ASC) and filter paper. Scanning electron microscopic studies of filter paper treated with Fn3(1,2), Fn3(1,2)-CBDIII, or CBDIII showed that the surface of the cellulose fibers had been loosened up and crenellated by Fn3(1,2) and Fn3(1,2)-CBDIII and to a lesser extent by CBDIII. X-ray diffraction analysis did not reveal changes in the crystallinity of the filter paper. CBDIII bound to ASC and filter paper with capacities of 2.45 and 0.73 micro moles g(-1) and relative affinities (K(r)) of 1.12 and 2.13 liters g(-1), respectively. Fn3(1,2) bound weakly to both celluloses. Fn3(1,2)-CBD bound to ASC and filter paper with capacities of 3.22 and 0.81 micro moles g(-1) and K(r)s of 1.14 and 1.98 liters g(-1), respectively. Fn3(1,2) and CBDIII contained 2 and 1 mol of calcium per mol, respectively. The results suggest that Fn3(1,2) aids the hydrolysis of cellulose by modifying its surface. This effect is enhanced by the presence of CBDIII, which increases the concentration of Fn3(1,2) on the cellulose surface.
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