ArticleApplied and environmental microbiology2002
Expression of genes encoding F(1)-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- Manganese Modulates Metabolic Activity and Redox Homeostasis in Translationally BlockedMicrobiology spectrum · 2022Article
- Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum.Microbial cell factories · 2021Article
- Article
- ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production.Biotechnology for biofuels · 2020Article
- Respiratory Physiology of Lactococcus lactis in Chemostat Cultures and Its Effect on Cellular Robustness in Frozen and Freeze-Dried Starter Cultures.Applied and environmental microbiology · 2020Article
- Genetics of Lactococci.Microbiology spectrum · 2019Review
- Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.Frontiers in microbiology · 2019Review
- Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case ofFrontiers in bioengineering and biotechnology · 2019Review
- Systems biology of lactic acid bacteria: For food and thought.Current opinion in systems biology · 2017Review
- A novel cell factory for efficient production of ethanol from dairy waste.Biotechnology for biofuels · 2016Article
- Physiological and Transcriptional Responses of Different Industrial Microbes at Near-Zero Specific Growth Rates.Applied and environmental microbiology · 2015Review
- Molecular and metabolic adaptations of Lactococcus lactis at near-zero growth rates.Applied and environmental microbiology · 2015Article
- Article
- Oxidative stress at high temperatures in Lactococcus lactis due to an insufficient supply of Riboflavin.Applied and environmental microbiology · 2013Article
- Systems biology of lactic acid bacteria: a critical review.Microbial cell factories · 2011Review
- Functional analysis of the role of CggR (central glycolytic gene regulator) in Lactobacillus plantarum by transcriptome analysis.Microbial biotechnology · 2011Article
- Understanding the physiology of Lactobacillus plantarum at zero growth.Molecular systems biology · 2010Article
- Metabolic control analysis: a tool for designing strategies to manipulate metabolic pathways.Journal of biomedicine & biotechnology · 2008Review
- The las enzymes control pyruvate metabolism in Lactococcus lactis during growth on maltose.Journal of bacteriology · 2007Article
- The pool of ADP and ATP regulates anaerobic product formation in resting cells of Lactococcus lactis.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied how the introduction of an additional ATP-consuming reaction affects the metabolic fluxes in Lactococcus lactis. Genes encoding the hydrolytic part of the F(1) domain of the membrane-bound (F(1)F(0)) H(+)-ATPase were expressed from a range of synthetic constitutive promoters. Expression of the genes encoding F(1)-ATPase was found to decrease the intracellular energy level and resulted in a decrease in the growth rate. The yield of biomass also decreased, which showed that the incorporated F(1)-ATPase activity caused glycolysis to be uncoupled from biomass production. The increase in ATPase activity did not shift metabolism from homolactic to mixed-acid fermentation, which indicated that a low energy state is not the signal for such a change. The effect of uncoupled ATPase activity on the glycolytic flux depended on the growth conditions. The uncoupling stimulated the glycolytic flux threefold in nongrowing cells resuspended in buffer, but in steadily growing cells no increase in flux was observed. The latter result shows that glycolysis occurs close to its maximal capacity and indicates that control of the glycolytic flux under these conditions resides in the glycolytic reactions or in sugar transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.