ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2002
Active, but not inactive, human centromeres display topoisomerase II activity in vivo.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- DNA strand breaks at centromeres: Friend or foe?Seminars in cell & developmental biology · 2024Review
- DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function.eLife · 2022Article
- Review
- Review
- Site-Specific Cleavage by Topoisomerase 2: A Mark of the Core Centromere.International journal of molecular sciences · 2018Article
- Non-Catalytic Roles of the Topoisomerase IIα C-Terminal Domain.International journal of molecular sciences · 2017Review
- Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei.Nucleic acids research · 2011Article
- PIASy-dependent SUMOylation regulates DNA topoisomerase IIalpha activity.The Journal of cell biology · 2010Article
- The evolutionary origin of man can be traced in the layers of defunct ancestral alpha satellites flanking the active centromeres of human chromosomes.PLoS genetics · 2009Article
- Review
- Hairpin structures formed by alpha satellite DNA of human centromeres are cleaved by human topoisomerase IIalpha.Nucleic acids research · 2008Article
- Kinetochore structure and spindle assembly checkpoint signaling in the budding yeast, Saccharomyces cerevisiae.Frontiers in bioscience : a journal and virtual library · 2008Review
- Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads.Journal of cell science · 2007Article
- Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi.Genome biology · 2007Article
- Evidence on the chromosomal location of centromeric DNA in Plasmodium falciparum from etoposide-mediated topoisomerase-II cleavage.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Progressive proximal expansion of the primate X chromosome centromere.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Topoisomerase II cleavage activity within the human D11Z1 and DXZ1 alpha-satellite arrays.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Article
- Centromeric protein bodies on avian lampbrush chromosomes contain a protein detectable with an antibody against DNA topoisomerase II.Chromosoma · 2004Article
- Construction, characterization, and complementation of a conditional-lethal DNA topoisomerase IIalpha mutant human cell line.Molecular biology of the cell · 2004Article
- Topoisomerase II: untangling its contribution at the centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic centromeres are composed of centromere DNA and the multiple proteins directly or indirectly associated with it. One important DNA-binding protein in the centromere is DNA topoisomerase II (topo II). In the genome in general, topo II has two functions, one structural and one enzymatic, the latter catalyzing DNA strand-passage reactions. It has been demonstrated that topo II accumulates at centromeres during the first part of mitosis, and disappears again at anaphase, but it has not been clear whether it serves a structural or an enzymatic function at the centromere. To investigate this issue, we developed the topo II-induced self-primed in situ assay (Topo-SPRINS). In this assay, DNA breaks created by topo II are stabilized with the topo II inhibitor VM-26 in vivo, and used as 'primers' for localized DNA synthesis in vitro. The assay revealed that topo II has enzymatic activity at mitotic centromeres and that the activity is relatively constant across centromeres. Furthermore, the activity was observed at a neocentromere, and, in multicentric chromosomes, the activity was restricted to the active centromere. The topo II activity is thus selectively present at functioning centromeres, indicating that it plays a role in mitotic centromere function.
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