ArticleThe EMBO journal2002
Crystal structures of transcription factor NusG in light of its nucleic acid- and protein-binding activities.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 115 citations in OpenAlex.
- Development of triaryl antimicrobials by scaffold hopping from an aminopropanol hit targeting bacterial RNA polymerase-NusG interactions.Journal of enzyme inhibition and medicinal chemistry · 2025Article
- Applying the brakes to transcription: regulation of gene expression by RNA polymerase pausing.Journal of bacteriology · 2025Review
- NusG-Spt5 Transcription Factors: Universal, Dynamic Modulators of Gene Expression.Journal of molecular biology · 2025Review
- Many dissimilar NusG protein domains switch between α-helix and β-sheet folds.Nature communications · 2022Article
- Metamorphic proteins under a computational microscope: Lessons from a fold-switching RfaH protein.Computational and structural biotechnology journal · 2022Review
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Metamorphic proteins: the Janus proteins of structural biology.Open biology · 2021Article
- Functional and Regulatory Roles of Fold-Switching Proteins.Structure (London, England : 1993) · 2021Review
- Article
- Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid-liquid phase separation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biophysical characterization and molecular phylogeny of human KIN protein.European biophysics journal : EBJ · 2019Article
- Two Old Dogs, One New Trick: A Review of RNA Polymerase and Ribosome Interactions during Transcription-Translation Coupling.International journal of molecular sciences · 2019Review
- Unfolding the Mysteries of Protein Metamorphosis.ACS chemical biology · 2018Review
- The PFrontiers in molecular biosciences · 2018Review
- Flipping states: a few key residues decide the winning conformation of the only universally conserved transcription factor.Nucleic acids research · 2017Article
- Transcription factors that influence RNA polymerases I and II: To what extent is mechanism of action conserved?Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- Structural insights into NusG regulating transcription elongation.Nucleic acids research · 2017Article
- Thermotoga maritima NusG: domain interaction mediates autoinhibition and thermostability.Nucleic acids research · 2017Article
- NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble.eLife · 2016Article
- The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.Protein science : a publication of the Protein Society · 2016Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial transcription modulator NusG interacts with RNA polymerase and termination factor rho, displaying striking functional homology to eukaryotic Spt5. The protein is also a translational regulator. We have determined crystal structures of Aquifex aeolicus NusG showing a modular design: an N-terminal RNP-like domain, a C-terminal element with a KOW sequence motif and a species-specific immunoglobulin-like fold. The structures reveal bona fide nucleic acid binding sites, and nucleic acid binding activities can be detected for NusG from three organisms and for the KOW element alone. A conserved KOW domain is defined as a new class of nucleic acid binding folds. This module is a close structural homolog of tudor protein-protein interaction motifs. Putative protein binding sites for the RNP and KOW domains can be deduced, which differ from the areas implicated in nucleic acid interactions. The results strongly argue that both protein and nucleic acid contacts are important for NusG's functions and that the factor can act as an adaptor mediating indirect protein-nucleic acid associations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.