ArticleThe EMBO journal2002
Tyrosine 221 in Crk regulates adhesion-dependent membrane localization of Crk and Rac and activation of Rac signaling.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 75 citations in OpenAlex.
- Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infection.Nature communications · 2025Article
- Adaptor protein Abelson interactor 1 in homeostasis and disease.Cell communication and signaling : CCS · 2024Review
- Article
- Lack of Paxillin phosphorylation promotes single-cell migration in vivo.The Journal of cell biology · 2023Article
- Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway.Cytotechnology · 2022Article
- Optimal Drift Correction for Superresolution Localization Microscopy with Bayesian Inference.Biophysical journal · 2015Article
- The proto-oncogene c-Src and its downstream signaling pathways are inhibited by the metastasis suppressor, NDRG1.Oncotarget · 2015Article
- p104 binds to Rac1 and reduces its activity during myotube differentiation of C2C12 cell.TheScientificWorldJournal · 2014Article
- Lck tyrosine kinase mediates β1-integrin signalling to regulate Schwann cell migration and myelination.Nature communications · 2013Article
- Crk adaptor proteins act as key signaling integrators for breast tumorigenesis.Breast cancer research : BCR · 2012Article
- Staged-probability strategy of processing shotgun proteomic data to discover more functionally important proteins.Protein & cell · 2012Article
- Phosphorylation of Crk on tyrosine 251 in the RT loop of the SH3C domain promotes Abl kinase transactivation.Oncogene · 2011Article
- CrkII transgene induces atypical mammary gland development and tumorigenesis.The American journal of pathology · 2010Article
- Regulation of cell migration and morphogenesis by Abl-family kinases: emerging mechanisms and physiological contexts.Journal of cell science · 2009Article
- Crk and CrkL adaptor proteins: networks for physiological and pathological signaling.Cell communication and signaling : CCS · 2009Article
- Abelson tyrosine kinase facilitates Salmonella enterica serovar Typhimurium entry into epithelial cells.Infection and immunity · 2009Article
- Article
- A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors.Journal of cell science · 2008Article
- The Rsu-1-PINCH1-ILK complex is regulated by Ras activation in tumor cells.European journal of cell biology · 2008Article
- RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization.PLoS pathogens · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The adaptor protein CrkII plays a central role in signal transduction cascades downstream of a number of different stimuli. We and others have previously shown that CrkII mediates attachment-induced JNK activation, membrane ruffling and cell motility in a Rac-dependent manner. We report here that cell attachment leads to tyrosine phosphorylation of CrkII on Y221, and that CrkII-Y221F mutant demonstrates enhanced association with the Crk-binding partners C3G and paxillin. Despite this enhanced signaling complex formation, CrkII-Y221F fails to induce JNK and PAK activation, membrane ruffling and cell migration, suggesting that it is defective in activating Rac signaling. Wild-type CrkII has no effect on adhesion-induced GTP loading of Rac, but its expression results in enhanced membrane localization of Rac, which is known to be required for Rac signaling. In contrast, CrkII-Y221F is deficient in enhancing membrane localization of Rac. Mutations in Rac and CrkII-Y221F that force membrane targeting of these molecules restore Rac signaling in adherent cells. Together, these results indicate that the Y221 site in CrkII regulates Rac membrane translocation upon cell adhesion, which is necessary for activation of downstream Rac signaling pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.