ArticleThe EMBO journal2002
Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- GDI2 protein: research progress and its mechanisms in diseases.Frontiers in cell and developmental biology · 2026Review
- RhoGDI1 regulates cell-cell junctions in polarized epithelial cells.Frontiers in cell and developmental biology · 2024Article
- The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery.The Journal of cell biology · 2022Article
- The role of the Siglec-G ITIM domain during bacterial infection.Cellular and molecular biology (Noisy-le-Grand, France) · 2022Article
- Article
- Review
- Convergent Evolution of the Osmoregulation System in Decapod Shrimps.Marine biotechnology (New York, N.Y.) · 2017Article
- RhoGDIβ Inhibits Bone Morphogenetic Protein 4 (BMP4)-induced Adipocyte Lineage Commitment and Favors Smooth Muscle-like Cell Differentiation.The Journal of biological chemistry · 2015Article
- Sex differences in the acute in vivo effects of different human SP-A variants on the mouse alveolar macrophage proteome.Journal of proteomics · 2014Article
- Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells.International journal of oncology · 2013Article
- Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A.Proteome science · 2012Article
- Rab8a regulates the exocyst-mediated kiss-and-run discharge of the Dictyostelium contractile vacuole.Molecular biology of the cell · 2012Article
- In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement.Proteome science · 2011Article
- Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.American journal of respiratory cell and molecular biology · 2010Article
- Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases.BMC microbiology · 2009Article
- The Cryptococcus neoformans Rho-GDP dissociation inhibitor mediates intracellular survival and virulence.Infection and immunity · 2008Article
- The androgen and progesterone receptors regulate distinct gene networks and cellular functions in decidualizing endometrium.Endocrinology · 2008Article
- Probing mechanisms of axonopathy. Part I: Protein targets of 1,2-diacetylbenzene, the neurotoxic metabolite of aromatic solvent 1,2-diethylbenzene.Toxicological sciences : an official journal of the Society of Toxicology · 2008Article
- Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii.BMC microbiology · 2008Article
- The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rho GDP-dissociation inhibitors (RhoGDIs) modulate the cycling of Rho GTPases between active GTP-bound and inactive GDP-bound states. We identified two RhoGDI homologues in DICTYOSTELIUM: GDI1 shares 51-58% similarity to RhoGDIs from diverse species. GDI2 is more divergent (40-44% similarity) and lacks the N-terminal regulatory arm characteristic for RhoGDI proteins. Both are cytosolic proteins and do not relocalize upon reorganization of the actin cytoskeleton. Using a two-hybrid approach, we identified Rac1a/1b/1c, RacB, RacC and RacE as interacting partners for GDI1. Cells lacking GDI1 are multinucleate, grow slowly and display a moderate pinocytosis defect, but rates of phagocytosis are unaffected. Mutant cells present prominent actin-rich protrusions, and large vacuoles that are continuous with the contractile vacuole system. The actin polymerization response upon stimulation with cAMP was reduced, but the motile behavior toward the chemoattractant was unaffected. Our results indicate that GDI1 plays a central role in the regulation of signal transduction cascades mediated by Rho GTPases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.