ArticleThe EMBO journal2002
Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 86 citations in OpenAlex.
- A CDC20 allelic series reveals stage-specific control of metaphase-to-anaphase transitions during mitosis and meiosis in rice.Science China. Life sciences · 2026Article
- USP8 and Hsp70 regulate endoreplication by synergistically promoting Fzr deubiquitination and stabilization.Science advances · 2025Article
- Critical domains for NACC2-NTRK2 fusion protein activation.PloS one · 2024Article
- Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis.Frontiers in cell and developmental biology · 2024Review
- Mad2B forms a complex with Cdc20, Cdc27, Rev3 and Rev1 in response to cisplatin-induced DNA damage.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2023Article
- Fzr regulates silk gland growth by promoting endoreplication and protein synthesis in the silkworm.PLoS genetics · 2023Article
- Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.Communications biology · 2022Article
- Initial characterization of gap phase introduction in every cell cycle ofFrontiers in cell and developmental biology · 2022Article
- Phosphorylation of the Anaphase Promoting Complex activator FZR1/CDH1 is required for Meiosis II entry in mouse male germ cell.Scientific reports · 2020Article
- A novel transcriptional cascade is involved in Fzr-mediated endoreplication.Nucleic acids research · 2020Article
- Cell cycle oscillators underlying orderly proteolysis of E2F8.Molecular biology of the cell · 2020Article
- APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway.Bioscience reports · 2016Review
- Substrate Recognition by the Cdh1 Destruction Box Receptor Is a General Requirement for APC/CCdh1-mediated Proteolysis.The Journal of biological chemistry · 2016Article
- Purifying Cytokinetic Cells from an Asynchronous Population.Scientific reports · 2015Article
- Degradation of Ndd1 by APC/C(Cdh1) generates a feed forward loop that times mitotic protein accumulation.Nature communications · 2015Article
- Casein kinase 1δ is an APC/C(Cdh1) substrate that regulates cerebellar granule cell neurogenesis.Cell reports · 2015Article
- Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans.Molecular microbiology · 2015Article
- Co-activator independent differences in how the metaphase and anaphase APC/C recognise the same substrate.Biology open · 2014Article
- APOLLON protein promotes early mitotic CYCLIN A degradation independent of the spindle assembly checkpoint.The Journal of biological chemistry · 2014Article
- The ubiquitin proteasome system - implications for cell cycle control and the targeted treatment of cancer.Biochimica et biophysica acta · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anaphase promoting complex/cyclosome (APC/C), activated by fzy and fzr, degrades cell cycle proteins that carry RXXL or KEN destruction boxes (d-boxes). APC/C substrates regulate sequential events and must be degraded in the correct order during mitosis and G(1). We studied how d-boxes determine APC/C(fzy)/APC/C(fzr) specificity and degradation timing. Cyclin B1 has an RXXL box and is degraded by both APC/C(fzy) and APC/C(fzr); fzy has a KEN box and is degraded by APC/C(fzr) only. We characterized the degradation of substrates with swapped d-boxes. Cyclin B1 with KEN was degraded by APC/C(fzr) only. Fzy with RXXL could be degraded by APC/C(fzy) and APC/C(fzr). Interestingly, APC/C(fzy)- but not APC/C(fzr)-specific degradation is highly dependent on the location of RXXL. We studied degradation of tagged substrates in real time and observed that APC/C(fzr) is activated in early G(1). These observations demonstrate how d-box specificities of APC/C(fzy) and APC/C(fzr), and the successive activation of APC/C by fzy and fzr, establish the temporal degradation pattern. Our observations can explain further why some endogenous RXXL substrates are degraded by APC/C(fzy), while others are restricted to APC/C(fzr).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.