ArticleThe EMBO journal2002
Vesicle transmembrane potential is required for translocation to the cytosol of externally added FGF-1.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Article
- Nuclear Fibroblast Growth Factor Receptor Signaling in Skeletal Development and Disease.Current osteoporosis reports · 2019Review
- Membrane-Associated, Not Cytoplasmic or Nuclear, FGFR1 Induces Neuronal Differentiation.Cells · 2019Article
- Nucleolin regulates phosphorylation and nuclear export of fibroblast growth factor 1 (FGF1).PloS one · 2014Article
- FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior.The Journal of cell biology · 2012Article
- Cellular internalization of quantum dots noncovalently conjugated with arginine-rich cell-penetrating peptides.Journal of nanoscience and nanotechnology · 2010Article
- Local delivery of a collagen-binding FGF-1 chimera to smooth muscle cells in collagen scaffolds for vascular tissue engineering.Biomaterials · 2010Article
- Increased protein stability of FGF1 can compensate for its reduced affinity for heparin.The Journal of biological chemistry · 2009Article
- Human rhinovirus type 2 uncoating at the plasma membrane is not affected by a pH gradient but is affected by the membrane potential.Journal of virology · 2009Article
- Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus.Molecular and cellular biology · 2008Article
- Nuclear trafficking of secreted factors and cell-surface receptors: new pathways to regulate cell proliferation and differentiation, and involvement in cancers.Cell communication and signaling : CCS · 2006Article
- Avian infectious bronchitis virus enters cells via the endocytic pathway.Advances in experimental medicine and biology · 2006Article
- Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1.Molecular biology of the cell · 2005Article
- Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism.Molecular biology of the cell · 2004Article
- Nuclear localization and function of polypeptide ligands and their receptors: a new paradigm for hormone specificity within the mammary gland?Breast cancer research : BCR · 2003Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Externally added fibroblast growth factor-1 (FGF-1) is capable of crossing cellular membranes to reach the cytosol and the nucleus in a number of cell types. We have monitored the translocation of the growth factor by two methods: phosphorylation of FGF-1, and prenylation of an FGF-1 mutant that contains a C-terminal prenylation signal. Inhibition of endosomal acidification by ammonium chloride or monensin did not block the translocation of FGF-1, whereas bafilomycin A1, a specific inhibitor of vacuolar proton pumps, blocked translocation completely. A combination of ionophores expected to dissipate the vesicular membrane potential (valinomycin plus monensin) also fully inhibited the translocation. The inhibition of translocation by bafilomycin A1 was overcome in the presence of monensin or nigericin, while ouabain blocked translocation under these conditions. The data indicate that translocation of FGF-1 to cytosol occurs from the lumen of intracellular vesicles possessing vacuolar proton pumps, and that a vesicular membrane potential is required. Apparently, activation of vesicular Na+/K+-ATPase by monensin or nigericin generates a membrane potential that can support translocation when the proton pump is blocked.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.