ArticleJournal of bacteriology2002
Differential expression and localization of Mn and Fe superoxide dismutases in the heterocystous cyanobacterium Anabaena sp. strain PCC 7120.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation.Biotechnology for biofuels and bioproducts · 2022Article
- Zero-Valent Iron Nanoparticles Induce Reactive Oxygen Species in the Cyanobacterium,ACS omega · 2021Article
- Timing the evolution of antioxidant enzymes in cyanobacteria.Nature communications · 2021Article
- Function of ORFC of the polyketide synthase gene cluster on fatty acid accumulation in Schizochytrium limacinum SR21.Biotechnology for biofuels · 2021Article
- Genome-Wide Identification and Bioinformatics Characterization of Superoxide Dismutases in the Desiccation-Tolerant CyanobacteriumFrontiers in microbiology · 2021Article
- Metalloproteins in the Biology of Heterocysts.Life (Basel, Switzerland) · 2019Review
- Molecular characterization and expression analysis of iron superoxide dismutase gene fromPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Development of a cooperative two-factor adaptive-evolution method to enhance lipid production and prevent lipid peroxidation inBiotechnology for biofuels · 2018Article
- Molecular Cloning and Biochemical Characterization of the Iron Superoxide Dismutase from the Cyanobacterium Nostoc punctiforme ATCC 29133 and Its Response to Methyl Viologen-Induced Oxidative Stress.Molecular biotechnology · 2015Article
- Membrane targeting of MnSOD is essential for oxidative stress tolerance of nitrogen-fixing cultures of Anabaena sp. strain PCC7120.Plant molecular biology · 2015Article
- Oxidative stress management in the filamentous, heterocystous, diazotrophic cyanobacterium, Anabaena PCC7120.Photosynthesis research · 2013Article
- Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii.The Plant cell · 2012Article
- Superoxide dismutases: ancient enzymes and new insights.FEBS letters · 2012Review
- Nitrogen status dependent oxidative stress tolerance conferred by overexpression of MnSOD and FeSOD proteins in Anabaena sp. strain PCC7120.Plant molecular biology · 2011Article
- PSORTdb--an expanded, auto-updated, user-friendly protein subcellular localization database for Bacteria and Archaea.Nucleic acids research · 2011Article
- Iso-superoxide dismutase in Deinococcus grandis, a UV resistant bacterium.Journal of microbiology (Seoul, Korea) · 2009Article
- OxyGene: an innovative platform for investigating oxidative-response genes in whole prokaryotic genomes.BMC genomics · 2008Article
- Purification and biochemical characterization of a superoxide dismutase from the soluble fraction of the cyanobacterium, Spirulina platensis.World journal of microbiology & biotechnology · 2007Article
- Localization and role of manganese superoxide dismutase in a marine diatom.Plant physiology · 2006Article
- Function of oxygen resistance proteins in the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris hildenborough.Journal of bacteriology · 2003Article
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Abstract
Superoxide dismutases (Sods) play very important roles in preventing oxidative damages in aerobic organisms. The nitrogen-fixing heterocystous cyanobacterium Anabaena sp. strain PCC 7120 has two Sod-encoding genes: a sodB, encoding a soluble iron-containing Sod (FeSod), and a sodA, encoding a manganese-containing Sod (MnSod). The FeSod was purified and characterized. A recombinant FeSod was also obtained by overproduction in Escherichia coli. Immunoblot study of the FeSod during induction of heterocyst differentiation showed that the cells produced six- to eightfold more FeSod 8 h after a shift from a nitrogen-replete condition to a nitrogen-depleted condition. However, the amount of FeSod protein in filaments with mature heterocysts was the same as that in filaments grown with combined nitrogen. Superoxide anion-generating chemicals such as methyl viologen did not induce upregulation of the sodB gene expression. The predicted preprotein of the sodA gene has a leader peptide and a motif for membrane attachment at the N terminus of the mature protein. Activity staining after gel electrophoresis of the purified thylakoid membranes showed that most of the MnSod in Anabaena sp. strain PCC 7120 was located on thylakoids toward the lumenal side. Expression of the sodA gene in E. coli shows that the leader peptide was required for its activity and the membrane localization of the MnSod. Northern hybridization detected one 0.82-kb transcript of sodA. The sodA gene was upregulated by methyl viologen, whereas its amount was unchanged during heterocyst differentiation. Immunoblotting and activity staining showed that isolated heterocysts contained a lower but still significant amount of FeSod, suggesting that its function is required in heterocysts. No MnSod was observed in isolated heterocysts. These results show that the two different Sod proteins have differentiated roles in Anabaena sp. strain PCC 7120.
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