ArticleJournal of bacteriology2002
Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- DksA-Dependent Stringent Stress Response Drives Virulence and Gastrointestinal Persistence ofbioRxiv : the preprint server for biology · 2026Article
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- Antibiotic Persister Cells in Acinetobacter baumannii: Overview of Molecular Mechanisms and Removal Strategies.Environmental microbiology · 2025Review
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- Article
- Awakening sleeper cells: a narrative review on bacterial magic spot synthetases as potential drug targets to overcome persistence.Current genetics · 2022Review
- Dissecting the Fitness Costs of Complex Mutations.Molecular biology and evolution · 2021Article
- TusA Is a Versatile Protein That Links Translation Efficiency to Cell Division in Escherichia coli.Journal of bacteriology · 2021Article
- Bacterial behavior in human blood reveals complement evaders with some persister-like features.PLoS pathogens · 2020Article
- Sugar-Phosphate Metabolism Regulates Stationary-Phase Entry and Stalk Elongation in Caulobacter crescentus.Journal of bacteriology · 2020Article
- Persistence of Intracellular Bacterial Pathogens-With a Focus on the Metabolic Perspective.Frontiers in cellular and infection microbiology · 2020Review
- Article
- Contact-dependent growth inhibition induces high levels of antibiotic-tolerant persister cells in clonal bacterial populations.The EMBO journal · 2018Article
- DksA and ppGpp Regulate the σJournal of bacteriology · 2018Article
- Stringent Response Regulators Contribute to Recovery from Glucose Phosphate Stress in Escherichia coli.Applied and environmental microbiology · 2017Article
- Article
- The i6A37 tRNA modification is essential for proper decoding of UUX-Leucine codons during rpoS and iraP translation.RNA (New York, N.Y.) · 2016Article
- Stress sigma factor RpoS degradation and translation are sensitive to the state of central metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Co-ordinated regulation of the extracytoplasmic stress factor, sigmaE, with other Escherichia coli sigma factors by (p)ppGpp and DksA may be achieved by specific regulation of individual holoenzymes.Molecular microbiology · 2014Article
- Circuitry linking the Csr and stringent response global regulatory systems.Molecular microbiology · 2011Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial sigma factor RpoS is strongly induced under a variety of stress conditions and during growth into stationary phase. Here, we used rpoS-lac fusions in Escherichia coli to investigate control acting at the level of RpoS synthesis, which is especially evident when cells approach stationary phase in rich medium. Previous work has shown that the small molecule ppGpp is required for normal levels of RpoS in stationary phase. Despite the attraction of a model in which the ppGpp level controls stationary-phase induction of RpoS, careful measurement of rpoS-lac expression in a mutant lacking ppGpp showed similar effects during both exponential growth and stationary phase; the main effect of ppGpp was on basal expression. In addition, a modest regulatory defect was associated with the mutant lacking ppGpp, delaying the time at which full expression was achieved by 2 to 3 h. Deletion analysis showed that the defect in basal expression was distributed over several sequence elements, while the regulatory defect mapped to the region upstream of the rpoS ribosome-binding site (RBS) that contains a cis-acting antisense element. A number of other genes that have been suggested as regulators of rpoS were tested, including dksA, dsrA, barA, ppkx, and hfq. With the exception of the dksA mutant, which had a modest defect in Luria-Bertani medium, none of these mutants was defective for rpoS stationary-phase induction. Even a short rpoS segment starting at 24 nucleotides upstream of the AUG initiation codon was sufficient to confer substantial stationary-phase regulation, which was mainly posttranscriptional. The effect of RBS-proximal sequence was independent of all known trans-acting factors, including ppGpp.
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