ArticleMolecular and cellular biology2002
Targeted histone acetylation at the yeast CUP1 promoter requires the transcriptional activator, the TATA boxes, and the putative histone acetylase encoded by SPT10.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen,Journal of fungi (Basel, Switzerland) · 2021Article
- The N-Terminal Tail of Histone H3 Regulates Copper Homeostasis in Saccharomyces cerevisiae.Molecular and cellular biology · 2021Article
- Article
- Single-Molecule Analysis Reveals Linked Cycles of RSC Chromatin Remodeling and Ace1p Transcription Factor Binding in Yeast.Molecular cell · 2018Article
- Regulation of histone gene expression in budding yeast.Genetics · 2012Review
- A polar barrier to transcription can be circumvented by remodeler-induced nucleosome translocation.Nucleic acids research · 2011Article
- Analysis of transcriptional activation at a distance in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes.Molecular cell · 2007Article
- Cooperative binding of the yeast Spt10p activator to the histone upstream activating sequences is mediated through an N-terminal dimerization domain.Nucleic acids research · 2007Article
- Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene.Molecular and cellular biology · 2006Article
- Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements.Molecular and cellular biology · 2005Article
- Yeast recombination enhancer is stimulated by transcription activation.Molecular and cellular biology · 2005Article
- Spt10-dependent transcriptional activation in Saccharomyces cerevisiae requires both the Spt10 acetyltransferase domain and Spt21.Molecular and cellular biology · 2004Article
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5 authors.
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Abstract
The relationship between chromatin remodeling and histone acetylation at the yeast CUP1 gene was addressed. CUP1 encodes a metallothionein required for cell growth at high copper concentrations. Induction of CUP1 with copper resulted in targeted acetylation of both H3 and H4 at the CUP1 promoter. Nucleosomes containing upstream activating sequences and sequences farther upstream were the targets for H3 acetylation. Targeted acetylation of H3 and H4 required the transcriptional activator (Ace1p) and the TATA boxes, suggesting that targeted acetylation occurs when TATA-binding protein binds to the TATA box or at a later stage in initiation. We have shown previously that induction results in nucleosome repositioning over the entire CUP1 gene, which requires Ace1p but not the TATA boxes. Therefore, the movement of nucleosomes occurring on CUP1 induction is independent of targeted acetylation. Targeted acetylation of both H3 and H4 also required the product of the SPT10 gene, which encodes a putative histone acetylase implicated in regulation at core promoters. Disruption of SPT10 was lethal at high copper concentrations and correlated with slower induction and reduced maximum levels of CUP1 mRNA. These observations constitute evidence for a novel mechanism of chromatin activation at CUP1, with a major role for the TATA box.
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