ArticleGenome biology2002
Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling.
Article in Genome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Dynamic single-cell transcriptomics reveals lsamp-guided neural network formation in male S. japonicum driving female reproduction.Nature communications · 2026Article
- Review
- Schistosoma mansoni Larval Extracellular Vesicle protein 1 (SmLEV1) is an immunogenic antigen found in EVs released from pre-acetabular glands of invading cercariae.PLoS neglected tropical diseases · 2021Article
- In Silico Analysis of Common Long Noncoding RNAs inJournal of tropical medicine · 2021Article
- Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni.Nature communications · 2020Article
- Use of kinase inhibitors against schistosomes to improve and broaden praziquantel efficacy.Parasitology · 2020Article
- Live imaging of collagen deposition during experimental hepatic schistosomiasis and recovery: a view on a dynamic process.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Transcriptomic analysis of male and female Schistosoma mekongi adult worms.Parasites & vectors · 2018Article
- Dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in Schistosoma japonicum.Nature communications · 2017Article
- Article
- Intake of Erythrocytes Required for Reproductive Development of Female Schistosoma japonicum.PloS one · 2015Article
- Synergy of Omeprazole and Praziquantel In Vitro Treatment against Schistosoma mansoni Adult Worms.PLoS neglected tropical diseases · 2015Article
- Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing.Parasites & vectors · 2014Article
- Sm10.3, a member of the micro-exon gene 4 (MEG-4) family, induces erythrocyte agglutination in vitro and partially protects vaccinated mice against Schistosoma mansoni infection.PLoS neglected tropical diseases · 2014Article
- Development of "-omics" research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis.Frontiers in microbiology · 2014Review
- Combinatory microarray and SuperSAGE analyses identify pairing-dependently transcribed genes in Schistosoma mansoni males, including follistatin.PLoS neglected tropical diseases · 2013Article
- Sex-biased expression of microRNAs in Schistosoma mansoni.PLoS neglected tropical diseases · 2013Article
- Genetic manipulation of schistosomes--progress with integration competent vectors.Parasitology · 2012Review
- Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host.PLoS neglected tropical diseases · 2011Article
- Differential expression of chemokine and matrix re-modelling genes is associated with contrasting schistosome-induced hepatopathology in murine models.PLoS neglected tropical diseases · 2011Article
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3 authors.
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Abstract
backgroundParasitic helminths of the genus Schistosoma mate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the infection are associated with this process. We have used cDNA microarray technology to initiate genome-wide gene-expression studies of sex and sexual development in mature Schistosoma mansoni parasites.
resultsAn S. mansoni-specific cDNA microarray was fabricated using 576 expressed sequence tags selected from three cDNA libraries and originating from two different parasite developmental stages. Five independent cDNA microarray hybridizations were analyzed using stringent filtering criteria and careful quality control, leading to the identification of 12 new female-associated and 4 new male-associated gene transcripts in the mature adult schistosome. Statistical analysis of variation demonstrated high levels of agreement within a cDNA microarray (correlation coefficient 0.91; median coefficient of variation 11.1%) and between cDNA microarrays (correlation coefficient 0.90; median coefficient of variation 14.4%). RT-PCR analysis confirmed the cDNA microarray results, thereby supporting the reliability of the system.
conclusionsOur study expands the list of S. mansoni gender-associated gene transcripts from all previous studies by a factor of two. Among the new associations identified, a tyrosinase ortholog was preferentially expressed in the adult female, and a dynein light-chain ortholog was highly induced in the adult male. cDNA microarrays offer the potential for exponential leaps in the understanding of parasite biology and this study shows how molecules involved in sexual biology can be rapidly identified.
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