ArticleOncogene2002
Rho-kinase contributes to diphosphorylation of myosin II regulatory light chain in nonmuscle cells.
Article in Oncogene, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 83 citations in OpenAlex.
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- Somatostatin peptides prevent increased human colonic epithelial permeability induced by hypoxia.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes inFrontiers in cell and developmental biology · 2022Article
- Drp1-mediated mitochondrial fission regulates calcium and F-actin dynamics during wound healing.Biology open · 2020Article
- Expression And Biological Interaction Network Of RHOC For Hepatic Carcinoma With Metastasis In PBMC Samples.OncoTargets and therapy · 2019Article
- Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1.The EMBO journal · 2018Article
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- Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue.Molecular biology of the cell · 2016Article
- Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption.Scientific reports · 2016Article
- Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells.Biochemical and biophysical research communications · 2015Article
- Activating transcription factor 5 enhances radioresistance and malignancy in cancer cells.Oncotarget · 2015Article
- Ibuprofen protects ventilator-induced lung injury by downregulating Rho-kinase activity in rats.BioMed research international · 2014Article
- Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding.The Journal of cell biology · 2013Article
- DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Article
- F-actin polymerization and retrograde flow drive sustained PLCγ1 signaling during T cell activation.The Journal of cell biology · 2012Article
- Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1).Molecular human reproduction · 2012Article
- Article
- Sequential activation of apical and basolateral contractility drives ascidian endoderm invagination.Current biology : CB · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Although the regulation of monophosphorylated MRLC at serine 19 throughout the cell cycle was examined in detail, MRLC diphosphorylation at both threonine 18 and serine 19 is still unclear. Here we found that Rho-kinase has an activity for MRLC diphosphorylation in nonmuscle cells using sequential column chromatographies. Transfection of Rho-kinase-EGFP induced the excess diphosphorylated MRLC and the bundling of the actin filaments. Conversely, the treatment of cells with a specific inhibitor of Rho-kinase, Y-27632, resulted in the decrease of endogenous diphosphorylated MRLC and actin stress fibers. Immunolocalization studies showed that both diphosphorylated MRLC and Rho-kinase accumulated and colocalized at the contractile ring and the midbody in dividing cells. Taken together, it is suggested that Rho-kinase contributes to MRLC diphosphorylation and reorganization of actin filaments in nonmuscle cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.