Evidence map›Paper›PMID 12184632›Full record

ArticleInflammation2002

Normal transcription of the C1 inhibitor gene is dependent upon a polypurine-polypyrimidine region within the promoter.

Kamyar Zahedi, Anne E Prada, Aideen Mulligan, Jorge A Prada, Alvin E Davis

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Kamyar ZahediDivision of Nephrology and Hypertension, Children's Hospital Research Foundation, Cincinnati, Ohio 45229, USA. zahek0@chmcc.org
Anne E Prada
Aideen Mulligan
Jorge A Prada
Alvin E Davis
Dana-Farber/Boston Children's Cancer and Blood Disorders Center · USHospital Research Foundation · AUCincinnati Children's Hospital Medical Center · US

Funding

C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMAR37HD022082 · NICHD · IMMUNE DISEASE INSTITUTE, INC. · PI DAVIS, ALVIN E · 1999 to 2008
$3.9M
REGULATION OF C1 INHIBITOR SYNTHESISR01HD033727 · NICHD · IMMUNE DISEASE INSTITUTE, INC. · PI DAVIS, ALVIN E · 1996 to 2004
$1.5M
THE C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMAR01HD022082 · NICHD · CHILDREN'S HOSPITAL MED CTR (CINCINNATI) · PI DAVIS, ALVIN E · 1987 to 1998
–
NICHD NIH HHS HD22082NICHD NIH HHS HD33727
6 · The paper itself

Abstract

Analysis of the transcriptional activity of C1 inhibitor (CIINH) promoter reporter constructs with mutations in the R-Y region indicate that triplex formation by this region is not a predictor of transcriptional activity and that normal promoter function depends on the interaction of trans acting factors with specific elements within this region. Electrophoretic mobility shift assay (EMSA) of Hep3B nuclear extracts using the wild type promoter probe (nucleotides -98 to -9) yielded four major bands. Incubation of the same extracts with probes lacking the HNF-1 site resulted in the disappearance of one band. Supershift assays indicate that HNF-1alpha is the only previously identified protein that is present in the EMSA bands. Southwestern blot analysis detected four bands (M(r)s -130, 75, 65 and 20 kDa). These data suggest that the -98 to -9 region of the C1INH promoter interacts with at least four proteins, one of which is HNF-1alpha.

Indexed as

Nuclear ProteinsTranscription, GeneticBlotting, SouthwesternComplement C1 Inactivator ProteinsComplement C1 Inhibitor ProteinDNA-Binding ProteinsElectrophoresisGene ExpressionHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaHumansMutationPromoter Regions, GeneticPurinesPyrimidinesComplement C1 Inactivator ProteinsComplement C1 Inhibitor ProteinDNA-Binding ProteinsHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaHNF1A protein, humanHNF1B protein, humanNuclear ProteinspurinePurinespyrimidinePyrimidinesSERPING1 protein, humanSerpinsTranscription Factors

Identifiers

PMID12184632
OpenAlexW1680937943

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.