ArticleInfection and immunity2002
Molecular genetic analyses of mating pheromones reveal intervariety mating or hybridization in Cryptococcus neoformans.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Cryptococcosis Serotypes Impact Outcome and Provide Evidence of Cryptococcus neoformans Speciation.mBio · 2015Article
- Cryptococcus gattii, no longer an accidental pathogen?Current fungal infection reports · 2012Article
- Heterozygosis and pathogenicity of Cryptococcus neoformans AD-hybrid isolates.Mycopathologia · 2012Article
- Genetic Diversity and Genomic Plasticity of Cryptococcus neoformans AD Hybrid Strains.G3 (Bethesda, Md.) · 2012Article
- Article
- Article
- Mitochondrial inheritance in haploid x non-haploid crosses in Cryptococcus neoformans.Current genetics · 2010Article
- Diploids in the Cryptococcus neoformans serotype A population homozygous for the alpha mating type originate via unisexual mating.PLoS pathogens · 2009Article
- Impact of mating type, serotype, and ploidy on the virulence of Cryptococcus neoformans.Infection and immunity · 2008Article
- Genetic analyses of a hybrid cross between serotypes A and D strains of the human pathogenic fungus Cryptococcus neoformans.Genetics · 2007Article
- alpha AD alpha hybrids of Cryptococcus neoformans: evidence of same-sex mating in nature and hybrid fitness.PLoS genetics · 2007Article
- Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State.BMC ophthalmology · 2007Article
- Microreview: capsule-associated genes of Cryptococcus neoformans.Mycopathologia · 2007Review
- Transcription factor STE12alpha has distinct roles in morphogenesis, virulence, and ecological fitness of the primary pathogenic yeast Cryptococcus gattii.Eukaryotic cell · 2006Article
- Cryptococcus gattii in AIDS patients, southern California.Emerging infectious diseases · 2005Article
- MFalpha1, the gene encoding the alpha mating pheromone of Candida albicans.Eukaryotic cell · 2003Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The sexual mating of the pathogenic yeast Cryptococcus neoformans is important for pathogenesis studies because the fungal virulence is linked to the alpha mating type (MAT(alpha)). We characterized C. neoformans mating pheromones (MF(alpha) 1 and MFa1) from 122 strains to understand intervariety hybridization or mating and intervariety virulence. MF(alpha) 1 in three C. neoformans varieties showed (a) specific nucleotide polymorphisms, (b) different copy numbers and chromosomal localizations, and (c) unique deduced amino acids in two geographic populations of C. neoformans var. gattii. MF(alpha) 1 of different varieties cross-hybridized in Southern hybridizations. Their phylogenetic analyses showed purifying selection (neutral evolution). These observations suggested that MAT(alpha) strains from any of the three C. neoformans varieties could mate or hybridize in nature with MATa strains of C. neoformans var. neoformans. A few serotype A/D diploid strains provided evidence for mating or hybridization, while a majority of A/D strains tested positive for haploid MF(alpha) 1 identical to that of C. neoformans var. grubii. MF(alpha) 1 sequence and copy numbers in diploids were identical to those of C. neoformans var. grubii, while their MFa1 sequences were identical to those of C. neoformans var. neoformans; thus, these strains were hybrids. The mice survival curves and histological lesions revealed A/D diploids to be highly pathogenic, with pathogenicity levels similar to that of the C. neoformans var. grubii type strain and unlike the low pathogenicity levels of C. neoformans var. neoformans strains. In contrast to MF(alpha) 1 in three varieties, MFa1 amplicons and hybridization signals could be obtained only from two C. neoformans var. neoformans reference strains and eight A/D diploids. This suggested that a yet undiscovered MFa pheromone(s) in C. neoformans var. gattii and C. neoformans var. grubii is unrelated to, highly divergent from, or rarer than that in C. neoformans var. neoformans. These observations could form the basis for future studies on the role of intervariety mating in C. neoformans biology and virulence.
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