ArticleInfection and immunity2002
Identification and characterization of a Brucella abortus ATP-binding cassette transporter homolog to Rhizobium meliloti ExsA and its role in virulence and protection in mice.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-Analysis and Advancement of Brucellosis Vaccinology.PloS one · 2016Pooled it
- A Meta-Analysis of the Protective Efficacy ofMicroorganisms · 2026Article
- Article
- Genomic and Immunoinformatics Insights Into a Bovine-Derived Brucella abortus S19 Field Strain: Adaptations Impacting Vaccine Efficacy.Veterinary medicine and science · 2025Article
- Characterization Studies on theVeterinary sciences · 2024Article
- Survival characteristics and transcriptome profiling reveal the adaptive response of theFrontiers in microbiology · 2022Article
- ATP-binding cassette (ABC) import systems ofEmerging microbes & infections · 2020Review
- Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.PloS one · 2020Article
- Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis.The Korean journal of parasitology · 2019Article
- Identification of Cross-Protective Potential Antigens against PathogenicJournal of immunology research · 2018Article
- The ABC transporter YejABEF is required for resistance to antimicrobial peptides and the virulence of Brucella melitensis.Scientific reports · 2016Article
- Varied metal-binding properties of lipoprotein PsaA in Streptococcus pneumoniae.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.PloS one · 2014Article
- Review
- Putative ATP-binding cassette transporter is essential for Brucella ovis pathogenesis in mice.Infection and immunity · 2011Article
- The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice.Infection and immunity · 2010Article
- Deletion of znuA virulence factor attenuates Brucella abortus and confers protection against wild-type challenge.Infection and immunity · 2006Article
- Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease.Journal of bacteriology · 2006Article
- Role of AFR1, an ABC transporter-encoding gene, in the in vivo response to fluconazole and virulence of Cryptococcus neoformans.Infection and immunity · 2006Article
- ATP-binding cassette transporters are targets for the development of antibacterial vaccines and therapies.Infection and immunity · 2004Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Brucella abortus is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. The mechanism of virulence of Brucella spp. is not fully understood yet. Furthermore, genes that allow Brucella to reach the intracellular niche and to interact with host cells need to be identified. Using the genomic survey sequence (GSS) approach, we identified the gene encoding an ATP-binding cassette (ABC) transporter of B. abortus strain S2308. The deduced amino acid sequence encoded by this gene exhibited 69 and 67% identity with the sequences of the ABC transporters encoded by the exsA genes of Rhizobium meliloti and Mesorhizobium loti, respectively. Additionally, B. abortus ExsA, like R. meliloti and M. loti ExsA, possesses ATP-binding motifs and the ABC signature domain features of a typical ABC transporter. Furthermore, ortholog group analysis placed B. abortus ExsA in ortholog group 6 of ABC transporters more likely to be involved in bacterial pathogenesis. In R. meliloti, ExsA is an exopolysaccharide transporter essential for alfalfa root nodule invasion and establishment of infection. To test the role of ExsA in Brucella pathogenesis, an exsA deletion mutant was constructed. Replacement of the wild-type exsA by recombination was demonstrated by Southern blot analysis of Brucella genomic DNA. Decreased survival in mice of the Brucella DeltaexsA mutant compared to the survival of parental strain S2308 demonstrated that ExsA is critical for full bacterial virulence. Additionally, the B. abortus exsA deletion mutant was used as a live vaccine. Challenge experiments revealed that the exsA mutant strain induced superior protective immunity in BALB/c mice compared to the protective immunity induced by strain S19 or RB51.
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