ArticleAntimicrobial agents and chemotherapy2002
Evaluation of antibiotic susceptibilities of three rickettsial species including Rickettsia felis by a quantitative PCR DNA assay.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 115 citations in OpenAlex.
- Rickettsiosis: existing advances and future opportunities for the development of vaccines.Veterinary research communications · 2026Review
- Comparison ofMicrobiology spectrum · 2026Article
- A repetitive nucleotide insertion in the rplV gene is associated with in vitro resistance to azithromycin in Rickettsia typhi.PLoS neglected tropical diseases · 2026Article
- Ectoparasite survey of schoolchildren in the Republic of Guinea.PLOS global public health · 2026Article
- Molecular identification and antibiotic clearance ofMicrobiology spectrum · 2025Article
- Reassessment of the genetic basis of natural rifampin resistance in the genus Rickettsia.MicrobiologyOpen · 2024Article
- Prediction of potential drug targets and key inhibitors (ZINC67974679, ZINC67982856, and ZINC05668040) againstFrontiers in veterinary science · 2024Article
- Identification of Vietnamese Flea Species and Their Associated Microorganisms Using Morphological, Molecular, and Protein Profiling.Microorganisms · 2023Article
- MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria.Microorganisms · 2022Article
- Detection of Tick-Borne Pathogens in Ticks from Cattle in Western Highlands of Cameroon.Microorganisms · 2022Article
- Article
- Buerger's Disease May be a Chronic Rickettsial Infection with Superimposed Thrombosis: Literature Review and Efficacy of Doxycycline in Three Patients.Infection & chemotherapy · 2022Review
- Multiple vector-borne pathogens of domestic animals in Egypt.PLoS neglected tropical diseases · 2021Article
- Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.PLoS neglected tropical diseases · 2021Article
- Article
- Molecular Characterization and Genetic Diversity of Haplogroup E Human Lice in Guinea, West Africa.Microorganisms · 2021Article
- Manifestations and Management of Flea-Borne Rickettsioses.Research and reports in tropical medicine · 2021Review
- Susceptibility ofThe American journal of tropical medicine and hygiene · 2019Article
- Anti-Mycoplasma Activity of Daptomycin and Its Use for Mycoplasma Elimination in Cell Cultures of Rickettsiae.Antibiotics (Basel, Switzerland) · 2019Article
- Rickettsial infections of the central nervous system.PLoS neglected tropical diseases · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rickettsiae grow only intracellularly, and the antibiotic susceptibilities of these bacteria have been assessed by either plaque, dye uptake, or immunofluorescence assays, which are time-consuming. We used a quantitative PCR (with the LightCycler instrument) to assess the levels of inhibition of Rickettisa felis, R. conorii, and R. typhi DNA synthesis in the presence of various antibiotics. We established the kinetics of rickettsial DNA during growth and showed that R. conorii grows more quickly than R. typhi in cell culture, with maximum replication occurring after 5 and 7 days, respectively. The MICs of the antibiotics tested for R. conorii and R. typhi by the quantitative PCR assay were similar to those previously obtained by plaque and dye uptake assays. We found that R. felis is susceptible to doxycycline, rifampin, thiamphenicol, and fluoroquinolones but not to gentamicin, erythromycin, amoxicillin, or trimethoprim-sulfamethoxazole. The resistance of this new species to erythromycin is consistent with its current taxonomic position within the spotted fever group. We believe that quantitative PCR could be used in the future to simplify and shorten antibiotic susceptibility assays of other rickettsiae and other strict intracellular pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.