ArticleProceedings of the National Academy of Sciences of the United States of America2002
High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 187 citations in OpenAlex.
- Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.Plant methods · 2024Article
- Insights into Mobile Small-RNAs Mediated Signaling in Plants.Plants (Basel, Switzerland) · 2022Review
- RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications.International journal of molecular sciences · 2022Review
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- The Mobile Small RNAs: Important Messengers for Long-Distance Communication in Plants.Frontiers in plant science · 2022Review
- Recent Advances in Plant Gene Silencing Methods.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Beyond identity: Understanding the contribution of the 5' nucleotide of the antisense strand to RNAi activity.PloS one · 2021Article
- Engineering DNA nanostructures for siRNA delivery in plants.Nature protocols · 2020Article
- The Whys and Wherefores of Transitivity in Plants.Frontiers in plant science · 2020Review
- A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction.Biotechnology for biofuels · 2019Article
- Transposon-associated epigenetic silencing during Pleurotus ostreatus life cycle.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
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- RNAi-mediated endogene silencing in strawberry fruit: detection of primary and secondary siRNAs by deep sequencing.Plant biotechnology journal · 2017Article
- miRNAs Do Not Regulate Circadian Protein Synthesis in the Dinoflagellate Lingulodinium polyedrum.PloS one · 2017Article
- Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus.Journal of experimental botany · 2014Article
- The Dictyostelium discoideum RNA-dependent RNA polymerase RrpC silences the centromeric retrotransposon DIRS-1 post-transcriptionally and is required for the spreading of RNA silencing signals.Nucleic acids research · 2014Article
- RNA-dependent RNA polymerase 6 is required for efficient hpRNA-induced gene silencing in plants.Molecules and cells · 2013Article
- Molecular modeling of metabolism for allergen-free low linoleic acid peanuts.Applied biochemistry and biotechnology · 2012Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranscriptional gene silencing (PTGS) in transgenic plants is an epigenetic form of RNA degradation related to PTGS and RNA interference (RNAi) in fungi and animals. Evidence suggests that transgene loci and RNA viruses can generate double-stranded RNAs similar in sequence to the transcribed region of target genes, which then undergo endonucleolytic cleavage to generate small interfering RNAs (siRNA) that promote degradation of cognate RNAs. The silent state in transgenic plants and in Caenorhabditis elegans can spread systemically, implying that mobile silencing signals exist. Neither the chemical nature of these signals nor their exact source in the PTGS pathway is known. Here, we use a positive marker system and real-time monitoring of green fluorescent protein expression to show that large sense, antisense, and double-stranded RNAs as well as double-stranded siRNAs delivered biolistically into plant cells trigger silencing capable of spreading locally and systemically. Systemically silenced leaves show greatly reduced levels of target RNA and accumulate siRNAs, confirming that RNA can induce systemic PTGS. The induced siRNAs represent parts of the target RNA that are outside of the region of homology with the triggering siRNA. Our results imply that siRNAs themselves or intermediates induced by siRNAs could comprise silencing signals and that these signals induce self-amplifying production of siRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.