ArticleMolecular biology of the cell2002
The MEK kinase Ssk2p promotes actin cytoskeleton recovery after osmotic stress.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- Stress contingent changes in Hog1 pathway architecture and regulation in Candida albicans.PLoS pathogens · 2024Article
- Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase.Biology · 2020Article
- Role of actin depolymerizing factor cofilin in Aspergillus fumigatus oxidative stress response and pathogenesis.Current genetics · 2018Article
- Network inference reveals novel connections in pathways regulating growth and defense in the yeast salt response.PLoS computational biology · 2018Article
- The septins FaCdc3 and FaCdc12 are required for cytokinesis and affect asexual and sexual development, lipid metabolism and virulence in Fusarium asiaticum.Molecular plant pathology · 2017Article
- Formaldehyde fixation is detrimental to actin cables in glucose-depletedMicrobial cell (Graz, Austria) · 2016Article
- The Stationary-Phase Cells of Saccharomyces cerevisiae Display Dynamic Actin Filaments Required for Processes Extending Chronological Life Span.Molecular and cellular biology · 2015Article
- Calcineurin regulates the yeast synaptojanin Inp53/Sjl3 during membrane stress.Molecular biology of the cell · 2015Article
- A comprehensive proteomic and phosphoproteomic analysis of yeast deletion mutants of 14-3-3 orthologs and associated effects of rapamycin.Proteomics · 2015Article
- Metabolic respiration induces AMPK- and Ire1p-dependent activation of the p38-Type HOG MAPK pathway.PLoS genetics · 2014Article
- Linking genetics to structural biology: complex heterozygosity screening with actin alanine scan alleles identifies functionally related surfaces on yeast actin.G3 (Bethesda, Md.) · 2014Article
- HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4).Cellular signalling · 2014Article
- Article
- Profiling lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and proteome microarrays.Molecular & cellular proteomics : MCP · 2012Article
- Response to hyperosmotic stress.Genetics · 2012Review
- The regulation of filamentous growth in yeast.Genetics · 2012Review
- The role of sho1 in polarized growth of Aspergillus fumigatus.Mycopathologia · 2011Article
- Diverse protective roles of the actin cytoskeleton during oxidative stress.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Article
- Network-free inference of knockout effects in yeast.PLoS computational biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Saccharomyces cerevisiae adapts to osmotic stress through the activation of a conserved high-osmolarity growth (HOG) mitogen-activated protein (MAP) kinase pathway. Transmission through the HOG pathway is very well understood, yet other aspects of the cellular response to osmotic stress remain poorly understood, most notably regulation of actin organization. The actin cytoskeleton rapidly disassembles in response to osmotic insult and is induced to reassemble only after osmotic balance with the environment is reestablished. Here, we show that one of three MEK kinases of the HOG pathway, Ssk2p, is specialized to facilitate actin cytoskeleton reassembly after osmotic stress. Within minutes of cells' experiencing osmotic stress or catastrophic disassembly of the actin cytoskeleton through latrunculin A treatment, Ssk2p concentrates in the neck of budding yeast cells and concurrently forms a 1:1 complex with actin. These observations suggest that Ssk2p has a novel, previously undescribed function in sensing damage to the actin cytoskeleton. We also describe a second function for Ssk2p in facilitating reassembly of a polarized actin cytoskeleton at the end of the cell cycle, a prerequisite for efficient cell cycle completion. Loss of Ssk2p, its kinase activity, or its ability to localize and interact with actin led to delays in actin recovery and a resulting delay in cell cycle completion. These unique capabilities of Ssk2p are activated by a novel mechanism that does not involve known components of the HOG pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.