ArticleMolecular biology of the cell2002
Fluorescence resonance energy transfer detection of synaptophysin I and vesicle-associated membrane protein 2 interactions during exocytosis from single live synapses.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- The function of VAMP2 in mediating membrane fusion: An overview.Frontiers in molecular neuroscience · 2022Review
- The MAL Protein, an Integral Component of Specialized Membranes, in Normal Cells and Cancer.Cells · 2021Review
- A comparative analysis of the mobility of 45 proteins in the synaptic bouton.The EMBO journal · 2020Article
- Activity ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Amyloid-β acts as a regulator of neurotransmitter release disrupting the interaction between synaptophysin and VAMP2.PloS one · 2012Article
- Synaptophysin is required for synaptobrevin retrieval during synaptic vesicle endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Article
- Lot1 is a key element of the pituitary adenylate cyclase-activating polypeptide (PACAP)/cyclic AMP pathway that negatively regulates neuronal precursor proliferation.The Journal of biological chemistry · 2009Article
- Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2.The Biochemical journal · 2007Article
- Determinants of synaptobrevin regulation in membranes.Molecular biology of the cell · 2007Article
- Structure of synaptophysin: a hexameric MARVEL-domain channel protein.Structure (London, England : 1993) · 2007Article
- Insecticidal toxins from black widow spider venom.Toxicon : official journal of the International Society on Toxinology · 2007Review
- Tactile stimulation-induced rapid elevation of the synaptophysin mRNA expression level in rat somatosensory cortex.Molecular and cellular biochemistry · 2006Article
- Alpha-herpesvirus glycoprotein D interaction with sensory neurons triggers formation of varicosities that serve as virus exit sites.The Journal of cell biology · 2006Article
- Tyrosine phosphorylation of synaptophysin in synaptic vesicle recycling.Biochemical Society transactions · 2005Review
- Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.Molecular biology of the cell · 2003Article
- Regulated delivery of AMPA receptor subunits to the presynaptic membrane.The EMBO journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants fused to synaptophysin I or VAMP2 were expressed in rat hippocampal neurons. We show that synaptophysin I and VAMP2 form both homo- and hetero-oligomers on the synaptic vesicle membrane. When exocytosis is stimulated with alpha-latrotoxin, VAMP2 dissociates from synaptophysin I even in the absence of appreciable exocytosis, whereas synaptophysin I oligomers disassemble only upon incorporation of the vesicle with the plasma membrane. We propose that synaptophysin I has multiple roles in neurotransmitter release, regulating VAMP2 availability for the soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex and possibly participating in the late steps of exocytosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.