ArticleMolecular biology of the cell2002
Multiple functions of sterols in yeast endocytosis.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 185 citations in OpenAlex.
- Structure of ergosteryl-aspartate synthase reveals how an entrapped tRNA is used like a prosthetic swinging arm in the synthesis of aminoacylated sterols.Nature communications · 2026Article
- Gim3 buffers and potentiates de novo mutations that affect fluconazole susceptibility in yeast.EMBO reports · 2026Article
- Deep learning reveals endogenous sterols as allosteric modulators of the GPCR-Gα interface.eLife · 2025Article
- Impact of ERG6 Gene Deletion on Membrane Composition and Properties in the Pathogenic Yeast Candida glabrata.Cell biochemistry and biophysics · 2025Article
- A geranylgeranyl reductase homolog required for cholesterol production in Myxococcota.Journal of bacteriology · 2025Article
- Ergosterol depletion by fish AMP analogs likely enhances fungal membrane permeability.Biophysical journal · 2025Article
- The evolution of antifungal therapy: Traditional agents, current challenges and future perspectives.Current research in microbial sciences · 2025Review
- Sterol-Targeted Laboratory Evolution Allows the Isolation of Thermotolerant and Respiratory-Competent Clones of the Industrial Yeast Saccharomyces cerevisiae.Microbial biotechnology · 2025Article
- Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.iScience · 2024Article
- Phytoconstituents and Ergosterol Biosynthesis-Targeting Antimicrobial Activity of Nutmeg (Molecules (Basel, Switzerland) · 2024Article
- Human Sterols Are Overproduced, Stored and Excreted in Yeasts.International journal of molecular sciences · 2024Article
- Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin.Scientific reports · 2023Article
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.Molecular biology of the cell · 2021Article
- Antioxidant Properties of Ergosterol and Its Role in Yeast Resistance to Oxidation.Antioxidants (Basel, Switzerland) · 2021Article
- Evolution of bacterial steroid biosynthesis and its impact on eukaryogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sterol Sponge Mechanism Is Conserved for Glycosylated Polyene Macrolides.ACS central science · 2021Article
- Article
- Understanding the Impact of Industrial Stress Conditions on Replicative Aging inFrontiers in fungal biology · 2021Review
- Plasma Membrane Fusion Is Specifically Impacted by the Molecular Structure of Membrane Sterols During Vegetative Development ofGenetics · 2020Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sterols are essential factors for endocytosis in animals and yeast. To investigate the sterol structural requirements for yeast endocytosis, we created a variety of ergDelta mutants, each accumulating a distinct set of sterols different from ergosterol. Mutant erg2Deltaerg6Delta and erg3Deltaerg6Delta cells exhibit a strong internalization defect of the alpha-factor receptor (Ste2p). Specific sterol structures are necessary for pheromone-dependent receptor hyperphosphorylation, a prerequisite for internalization. The lack of phosphorylation is not due to a defect in Ste2p localization or in ligand-receptor interaction. Contrary to most known endocytic factors, sterols seem to function in internalization independently of actin. Furthermore, sterol structures are required at a postinternalization step of endocytosis. ergDelta cells were able to take up the membrane marker FM4-64, but exhibited defects in FM4-64 movement through endosomal compartments to the vacuole. Therefore, there are at least two roles for sterols in endocytosis. Based on sterol analysis, the sterol structural requirements for these two processes were different, suggesting that sterols may have distinct functions at different places in the endocytic pathway. Interestingly, sterol structures unable to support endocytosis allowed transport of the glycosylphosphatidylinositol-anchored protein Gas1p from the endoplasmic reticulum to Golgi compartment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.