ArticleMolecular biology of the cell2002
Cytoplasmic localization of Wis1 MAPKK by nuclear export signal is important for nuclear targeting of Spc1/Sty1 MAPK in fission yeast.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Gamete fusion triggers cytosolic functions and P-body recruitment of the RNA-binding protein Mei2 to drive fission yeast zygotic development.Genes & development · 2026Article
- Fission yeast Cdc14-like phosphatase Flp1/Clp1 modulates the transcriptional response to oxidative stress.Scientific reports · 2023Article
- A new negative feedback mechanism for MAPK pathway inactivation through Srk1 MAPKAP kinase.Scientific reports · 2022Article
- Communication between Cyclin-dependent kinase Cdc2 and the Wis1-Spc1 MAPK pathway determines mitotic timing inBiology open · 2020Article
- A Redox-Sensitive Thiol in Wis1 Modulates the Fission Yeast Mitogen-Activated Protein Kinase Response to HMolecular and cellular biology · 2020Article
- Article
- IQGAP1: insights into the function of a molecular puppeteer.Molecular immunology · 2015Review
- Biological significance of nuclear localization of mitogen-activated protein kinase Pmk1 in fission yeast.The Journal of biological chemistry · 2012Article
- H₂O₂ stress-specific regulation of S. pombe MAPK Sty1 by mitochondrial protein phosphatase Ptc4.The EMBO journal · 2012Article
- Fission yeast MAP kinase Sty1 is recruited to stress-induced genes.The Journal of biological chemistry · 2008Article
- Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase.Molecular and cellular biology · 2008Article
- ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.Nature biotechnology · 2006Article
- Identification of Cdc37 as a novel regulator of the stress-responsive mitogen-activated protein kinase.Molecular and cellular biology · 2003Article
- A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway.The EMBO journal · 2003Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mitogen-activated protein kinase (MAPK) cascade is a ubiquitous signaling module that transmits extracellular stimuli through the cytoplasm to the nucleus; in response to activating stimuli, MAPKs translocate into the nucleus. Mammalian MEK MAPK kinases (MAPKKs) have in their N termini an MAPK-docking site and a nuclear export signal (NES) sequence, which are known to play critical roles in maintaining ERK MAPKs in the cytoplasm of unstimulated cells. Herein, we show that the Wis1 MAPKK of the stress-activated Spc1 MAPK cascade in fission yeast also has a MAPK-docking site and an NES sequence in its N-terminal domain. Unexpectedly, an inactivating mutation to the NES of chromosomal wis1(+) does not affect the subcellular localization of Spc1 MAPK, whereas this NES mutation disturbs the cytoplasmic localization of Wis1. However, when Wis1 is targeted to the nucleus by fusing to a nuclear localization signal sequence, stress-induced nuclear translocation of Spc1 is abrogated, indicating that cytoplasmic Wis1 is required for nuclear transport of Spc1 upon stress. Moreover, we have observed that a fraction of Wis1 translocates into the nucleus in response to stress. These results suggest that cytoplasmic localization of Wis1 MAPKK by its NES is important for stress signaling to the nucleus.
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