ArticleMolecular biology of the cell2002
Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Distinct TORC1 signalling branches regulate Adc17 proteasome assembly chaperone expression.Journal of cell science · 2024Article
- Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.Nature communications · 2019Article
- Identification of Suppressor of Clathrin Deficiency-1 (G3 (Bethesda, Md.) · 2019Article
- Endocytosis regulates TDP-43 toxicity and turnover.Nature communications · 2017Article
- Creating a chimeric clathrin heavy chain that functions independently of yeast clathrin light chain.Traffic (Copenhagen, Denmark) · 2016Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis.Molecular systems biology · 2014Article
- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Lessons from yeast for clathrin-mediated endocytosis.Nature cell biology · 2011Review
- Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin.Molecular biology of the cell · 2011Article
- Exo-endocytic trafficking and the septin-based diffusion barrier are required for the maintenance of Cdc42p polarization during budding yeast asymmetric growth.Molecular biology of the cell · 2011Article
- AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles.Molecular biology of the cell · 2009Article
- Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Scd5p mediates phosphoregulation of actin and endocytosis by the type 1 phosphatase Glc7p in yeast.Molecular biology of the cell · 2007Article
- PtdIns(4,5)P2 turnover is required for multiple stages during clathrin- and actin-dependent endocytic internalization.The Journal of cell biology · 2007Article
- Nucleocytoplasmic trafficking is required for functioning of the adaptor protein Sla1p in endocytosis.Traffic (Copenhagen, Denmark) · 2007Article
- Synthesis and function of membrane phosphoinositides in budding yeast, Saccharomyces cerevisiae.Biochimica et biophysica acta · 2007Review
- Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis.Molecular biology of the cell · 2007Article
- Novel function of clathrin light chain in promoting endocytic vesicle formation.Molecular biology of the cell · 2006Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
SCD5 was identified as a multicopy suppressor of clathrin HC-deficient yeast. SCD5 is essential, but an scd5-Delta338 mutant, expressing Scd5p with a C-terminal truncation of 338 amino acids, is temperature sensitive for growth. Further studies here demonstrate that scd5-Delta338 affects receptor-mediated and fluid-phase endocytosis and normal actin organization. The scd5-Delta338 mutant contains larger and depolarized cortical actin patches and a prevalence of G-actin bars. scd5-Delta338 also displays synthetic negative genetic interactions with mutations in several other proteins important for cortical actin organization and endocytosis. Moreover, Scd5p colocalizes with cortical actin. Analysis has revealed that clathrin-deficient yeast also have a major defect in cortical actin organization and accumulate G-actin. Overexpression of SCD5 partially suppresses the actin defect of clathrin mutants, whereas combining scd5-Delta338 with a clathrin mutation exacerbates the actin and endocytic phenotypes. Both Scd5p and yeast clathrin physically associate with Sla2p, a homologue of the mammalian huntingtin interacting protein HIP1 and the related HIP1R. Furthermore, Sla2p localization at the cell cortex is dependent on Scd5p and clathrin function. Therefore, Scd5p and clathrin are important for actin organization and endocytosis, and Sla2p may provide a critical link between clathrin and the actin cytoskeleton in yeast, similar to HIP1(R) in animal cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.