ArticleThe Journal of physiology2002
Excitation-contraction coupling and sarcoplasmic reticulum function in mechanically skinned fibres from fast skeletal muscles of aged mice.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Electrophysiological Evaluation of Post-Activation Potentiation/Post-Activation Performance Enhancement Using Strength-Duration Properties.Journal of functional morphology and kinesiology · 2026Article
- Article
- The Role of Osteokines in Sarcopenia: Therapeutic Directions and Application Prospects.Frontiers in cell and developmental biology · 2021Review
- Oxidative stress-induced dysregulation of excitation-contraction coupling contributes to muscle weakness.Journal of cachexia, sarcopenia and muscle · 2018Article
- Aging impairs regulation of ryanodine receptors from extensor digitorum longus but not soleus muscles.Muscle & nerve · 2018Article
- The NLRP3 inflammasome contributes to sarcopenia and lower muscle glycolytic potential in old mice.American journal of physiology. Endocrinology and metabolism · 2017Article
- Osteocalcin is necessary and sufficient to maintain muscle mass in older mice.Molecular metabolism · 2016Article
- Age-related functional changes and susceptibility to eccentric contraction-induced damage in skeletal muscle cell.Integrative medicine research · 2016Review
- Pinpointing beta adrenergic receptor in ageing pathophysiology: victim or executioner? Evidence from crime scenes.Immunity & ageing : I & A · 2013Article
- Age- and gender-related changes in contractile properties of non-atrophied EDL muscle.PloS one · 2010Article
- Cytoskeletal tropomyosin Tm5NM1 is required for normal excitation-contraction coupling in skeletal muscle.Molecular biology of the cell · 2009Article
- E-C coupling and contractile characteristics of mechanically skinned single fibres from young rats during rapid growth and maturation.Pflugers Archiv : European journal of physiology · 2008Article
- Sarcoplasmic reticulum Ca2+ release declines in muscle fibers from aging mice.Biophysical journal · 2008Article
- Changes in contractile activation characteristics of rat fast and slow skeletal muscle fibres during regeneration.The Journal of physiology · 2004Article
- Beta 2-agonist administration reverses muscle wasting and improves muscle function in aged rats.The Journal of physiology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ageing is generally associated with a decline in skeletal muscle mass and strength, and a slowing of muscle contraction, factors that impact upon the quality of life for the elderly. Alterations in Ca2+ handling are thought to contribute to these age-related changes in muscle contractility, yet the effects of ageing on sarcoplasmic reticulum (SR) Ca2+ handling and the Ca2+ transport system remain unresolved. We used mechanically skinned single fibres from the fast twitch extensor digitorum longus (EDL) muscles from young (4-month-old) and old (27- to 28-month-old) mice to test the hypothesis that the age-related changes in skeletal muscle contractility, especially the slower rate of contraction, are due to changes intrinsic to the muscle fibres. There were no age-related differences in the peak height of depolarization-induced contractile response (DICR) or the number of DICRs elicited before rundown (DICR < 50 % of initial). The time taken to reach peak DICR (TPDICR) was approximately12 % slower in single muscle fibres from old compared with young mice (P < 0.05). The rate of relaxation following DICR was not different in young and old mice. Examination of SR function demonstrated that SR Ca2+ reloading in Ca2+ -depleted skinned fibres was not different in young and old mice, nor was there any age-related difference in Ca2+ leak from the SR. However, low [caffeine] contracture in fibres from old mice was only half of that observed in fibres from young mice (P < 0.05), indicating a lower sensitivity of the SR Ca2+ release channel (CRC) to caffeine. We found no difference in maximum Ca2+ -activated force (P(o)) or specific force (sP(o); P(o) corrected for cross-sectional area) in EDL muscle fibres from young and old mice. Impaired excitation-contraction (E-C) coupling and a decrease in SR CRC function are mechanisms which are likely to contribute to the overall slowing of muscle contraction with age.
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