ArticlePlant physiology2002
Expression of a heterologous S-adenosylmethionine decarboxylase cDNA in plants demonstrates that changes in S-adenosyl-L-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- Physiological and molecular response mechanisms of tomato seedlings to cadmium (Cd) and lead (Pb) stress.PeerJ · 2024Article
- Identification of cucumber S-adenosylmethionine decarboxylase genes and functional analysis ofFrontiers in plant science · 2023Article
- Spermine Confers Stress Resilience by Modulating Abscisic Acid Biosynthesis and Stress Responses in Arabidopsis Plants.Frontiers in plant science · 2019Article
- Polyamines - A New Metabolic Switch: Crosstalk With Networks Involving Senescence, Crop Improvement, and Mammalian Cancer Therapy.Frontiers in plant science · 2019Article
- Polyamines in the life of Arabidopsis: profiling the expression of S-adenosylmethionine decarboxylase (SAMDC) gene family during its life cycle.BMC plant biology · 2017Article
- Spermidine affects the transcriptome responses to high temperature stress in ripening tomato fruit.Journal of Zhejiang University. Science. B · 2012Article
- Molecular characterization of the Arginine decarboxylase gene family in rice.Transgenic research · 2010Article
- Article
- Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase.Plant molecular biology · 2009Article
- Spermidine exodus and oxidation in the apoplast induced by abiotic stress is responsible for H2O2 signatures that direct tolerance responses in tobacco.The Plant cell · 2008Article
- Overexpression of carnation S-adenosylmethionine decarboxylase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants.Plant cell reports · 2006Article
- The Quest to Understand the Basis and Mechanisms that Control Expression of Introduced Transgenes in Crop Plants.Plant signaling & behavior · 2006Article
- The methylation cycle and its possible functions in barley endosperm development.Plant molecular biology · 2005Article
- Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants.Plant physiology · 2004Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cDNA involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. Transgenic rice (Oryza sativa) plants engineered with the heterologous Datura stramonium S-adenosylmethionine decarboxylase (samdc) cDNA exhibited accumulation of the transgene steady-state mRNA. Transgene expression did not affect expression of the orthologous samdc gene. Significant increases in SAMDC activity translated to a direct increase in the level of spermidine, but not spermine, in leaves. Seeds recovered from a number of plants exhibited significant increases in spermidine and spermine levels. We demonstrate that overexpression of the D. stramonium samdc cDNA in transgenic rice is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds. These findings suggest that increases in enzyme activity in one of the two components of the later parts of the pathway leading to the higher polyamines is sufficient to alter their levels mostly in seeds and, to some extent, in vegetative tissue such as leaves. Implications of our results on the design of rational approaches for the modulation of the polyamine pathway in plants are discussed in the general framework of metabolic pathway engineering.
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