ArticlePlant physiology2002
Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
132 citing papers in PubMed, 1 synthesis or guideline pooled it, 325 citations in OpenAlex.
- Meta-analysis of transcriptome reveals key genes relating to oil quality in olive.BMC genomics · 2023Pooled it
- TaLACS3 positively regulates wheat resistance to Puccinia striiformis f. sp. tritici by modulating ROS accumulation.BMC plant biology · 2026Article
- Genome-Wide Association Study Uncovers Candidate Genes Governing Oil Quality Traits in Sunflower (Plants (Basel, Switzerland) · 2026Article
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Identification, evolutionary diversification, and stress response roles of SmLACSs in Salix matsudana.BMC genomics · 2026Article
- Expression genome-wide association analysis (eGWAS) identifies a candidate gene influencing fatty acid composition in soybeans.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Evolutionary diversification of acyl-CoA synthetases underpins hydrophobic barrier formation across diverse tomato tissues and beyond.Horticulture research · 2025Article
- Study on the regulatory effects of Δ4-DES and LACS genes on the synthesis of LC-PUFAs, based on the transcriptome changes induced by fluridone in Aurantiochytrium limacinum OUC86.World journal of microbiology & biotechnology · 2025Article
- Proteomics revealed novel functions and drought tolerance of Arabidopsis thaliana protein kinase ATG1.BMC biology · 2025Article
- Identification and functional analysis ofFrontiers in plant science · 2025Article
- Transcriptional and metabolic analysis of oleic acid synthesis in seedless and tenera oil palm species.Frontiers in plant science · 2025Article
- Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.BMC plant biology · 2024Article
- Article
- Comparative Phenotypic and Transcriptomic Analyses Provide Novel Insights into the Molecular Mechanism of Seed Germination in Response to Low Temperature Stress in Alfalfa.International journal of molecular sciences · 2024Article
- Evolutionary origin and gradual accumulation with plant evolution of the LACS family.BMC plant biology · 2024Article
- Genome-wide expression analysis of LACS gene family implies GhLACS25 functional responding to salt stress in cotton.BMC plant biology · 2024Article
- The acyl-CoA synthetasemBio · 2024Article
- Transcriptomics aids in uncovering the metabolic shifts and molecular machinery of Schizochytrium limacinum during biotransformation of hydrophobic substrates to docosahexaenoic acid.Microbial cell factories · 2024Article
- Genomic Insights into High-Altitude Adaptation: A Comparative Analysis ofInternational journal of molecular sciences · 2024Article
72 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-chain acyl-coenzyme A (CoA) synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters and as such play critical roles in fatty acid metabolism. This important class of enzymes factors prominently in several fatty acid-derived metabolic pathways, including phospholipid, triacylglycerol, and jasmonate biosynthesis and fatty acid beta-oxidation. In an effort to better understand the factors that control fatty acid metabolism in oilseeds, we have sought to identify and characterize genes that encode LACSs in Arabidopsis. Nine cDNAs were identified, cloned, and tested for their ability to complement a LACS-deficient strain of yeast (Saccharomyces cerevisiae). Seven of the nine successfully restored growth, whereas two cDNAs encoding putative peroxisomal isoforms did not. Lysates from yeast cells overexpressing each of the nine cDNAs were active in LACS enzyme assays using oleic acid as a substrate. The substrate specificities of the enzymes were determined after overexpression in LACS-deficient Escherichia coli. Most of the LACS enzymes displayed highest levels of activity with the fatty acids that make up the common structural and storage lipids in Arabidopsis tissues. Analysis of the tissue-specific expression profiles for these genes revealed one flower-specific isoform, whereas all others were expressed in various tissues throughout the plant. These nine cDNAs are thought to constitute the entire LACS family in Arabidopsis, and as such, will serve as powerful tools in the study of acyl-CoA metabolism in oilseeds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.