ArticlePlant physiology2002
Overexpression of the clpP 5'-untranslated region in a chimeric context causes a mutant phenotype, suggesting competition for a clpP-specific RNA maturation factor in tobacco chloroplasts.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology.Biological research · 2022Article
- Construction of DNA Tools for Hyperexpression inACS synthetic biology · 2021Article
- Mitigation of deleterious phenotypes in chloroplast-engineered plants accumulating high levels of foreign proteins.Biotechnology for biofuels · 2021Article
- MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology.Plant physiology · 2019Article
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
- Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco.Journal of experimental botany · 2017Article
- The HIV-1 Pr55 gag polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants.Transgenic research · 2015Article
- Article
- Visual spectinomycin resistance (aadA(au)) gene for facile identification of transplastomic sectors in tobacco leaves.Plant molecular biology · 2011Article
- High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.Transgenic research · 2011Article
- Overexpression of a natural chloroplast-encoded antisense RNA in tobacco destabilizes 5S rRNA and retards plant growth.BMC plant biology · 2010Article
- Study of plastid genome stability in tobacco reveals that the loss of marker genes is more likely by gene conversion than by recombination between 34-bp loxP repeats.Plant physiology · 2010Article
- Genome-wide analysis of plastid gene expression in potato leaf chloroplasts and tuber amyloplasts: transcriptional and posttranscriptional control.Plant physiology · 2009Article
- Plant physiological adaptations to the massive foreign protein synthesis occurring in recombinant chloroplasts.Plant physiology · 2009Article
- High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts.Planta · 2009Article
- Disruption of essential plastid gene expression caused by T7 RNA polymerase-mediated transcription of plastid transgenes during early seedling development.Transgenic research · 2007Article
- Plastid marker gene excision by the phiC31 phage site-specific recombinase.Plant molecular biology · 2007Article
- Mutations in ClpC2/Hsp100 suppress the requirement for FtsH in thylakoid membrane biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Expression of tetanus toxin Fragment C in tobacco chloroplasts.Nucleic acids research · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastid ribosomal RNA (rrn) operon promoter was fused with DNA segments encoding the leader sequence (5'-untranslated region [UTR]) of plastid mRNAs to compare their efficiency in mediating translation of a bacterial protein neomycin phosphotransferase (NPTII) in tobacco (Nicotiana tabacum) chloroplasts. In young leaves, NPTII accumulated at 0.26% and 0.8% of the total soluble leaf protein from genes with the clpP and atpB 5'-UTR, respectively. Interestingly, expression of NPTII from the promoter with the clpP 5'-UTR (0.26% NPTII) caused a mutant (chlorotic) phenotype, whereas plants accumulating approximately 0.8% NPTII from the atpB 5'-UTR were normal green, indicating that the mutant phenotype was independent of NPTII accumulation. Low levels of monocistronic clpP mRNA and accumulation of intron-containing clpP transcripts in the chlorotic leaves suggest competition between the clpP 5'-UTR in the chimeric transcript and the native clpP pre-mRNA (ratio 16:1) for an mRNA maturation factor. Because maturation of 11 other intron-containing mRNAs was unaffected in the chlorotic leaves, it appears that the factor is clpP specific. The mutant phenotype is correlated with reduced levels (approximately 2 times) of the ClpP1 protease subunit, supporting an important role for ClpP1 in chloroplast development.
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