ArticlePlant physiology2002
Activation tagging using the En-I maize transposon system in Arabidopsis.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Review
- Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.Advanced genetics (Hoboken, N.J.) · 2024Article
- Genome-Wide Characterization and Haplotypic Variation Analysis of theInternational journal of molecular sciences · 2023Article
- Genomic Survey of Flavin Monooxygenases in Wild and Cultivated Rice Provides Insight into Evolution and Functional Diversities.International journal of molecular sciences · 2023Article
- Twisting development, the birth of a potential new gene.iScience · 2022Article
- The AP2 Transcription FactorInternational journal of molecular sciences · 2022Article
- ROS1-mediated decrease in DNA methylation and increase in expression of defense genes and stress response genes in Arabidopsis thaliana due to abiotic stresses.BMC plant biology · 2022Article
- De novo screening of disease-resistant genes from the chromosome-level genome of rare minnow using CRISPR-cas9 random mutation.GigaScience · 2021Article
- Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 inSaudi journal of biological sciences · 2021Article
- YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana.Genetics and molecular biology · 2020Article
- The Roles of Auxin Biosynthesis YUCCA Gene Family in Plants.International journal of molecular sciences · 2019Review
- Development of an activation tagging system for maize.Plant direct · 2019Article
- Overexpression of the DEAD-Box RNA Helicase GeneInternational journal of molecular sciences · 2018Article
- A low degenerate primer pool improved the efficiency of high-efficiency thermal asymmetric interlaced PCR to amplify T-DNA flanking sequences in3 Biotech · 2018Article
- Jasmonate-Sensitivity-Assisted Screening and Characterization of Nicotine Synthetic Mutants from Activation-Tagged Population of Tobacco (Frontiers in plant science · 2017Article
- A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis.Nature communications · 2015Article
- Characterization of a potato activation-tagged mutant, nikku, and its partial revertant.Planta · 2015Article
- High-throughput generation of an activation-tagged mutant library for functional genomic analyses in tobacco.Planta · 2015Article
- bHLH106 Integrates Functions of Multiple Genes through Their G-Box to Confer Salt Tolerance on Arabidopsis.PloS one · 2015Article
- Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance.Plant molecular biology · 2014Article
2 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method for the generation of stable activation tag inserts was developed in Arabidopsis using the maize (Zea mays) En-I transposon system. The method employs greenhouse selectable marker genes that are useful to efficiently generate large populations of insertions. A population of about 8,300 independent stable activation tag inserts has been produced. Greenhouse-based screens for mutants in a group of plants containing about 2,900 insertions revealed about 31 dominant mutants, suggesting a dominant mutant frequency of about 1%. From the first batch of about 400 stable insertions screened in the greenhouse, four gain-in-function, dominant activation-tagged, morphological mutants were identified. A novel gain-in-function mutant called thread is described, in which the target gene belongs to the same family as the YUCCA flavin-mono-oxygenase that was identified by T-DNA activation tagging. The high frequency of identified gain-in-function mutants in the population suggests that the En-I system described here is an efficient strategy to saturate plant genomes with activation tag inserts. Because only a small number of primary transformants are required to generate an activation tag population, the En-I system appears to be an attractive alternative to study plant species where the present transformation methods have low efficiencies.
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