ArticleNucleic acids research2002
Manufacturing DNA microarrays from unpurified PCR products.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications.Polymers · 2021Article
- A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.PloS one · 2014Article
- A vertically stacked, polymer, microfluidic point mutation analyzer: rapid high accuracy detection of low-abundance K-ras mutations.Analytical biochemistry · 2011Article
- Gene expression profiles characterize inflammation stages in the acute lung injury in mice.PloS one · 2010Article
- Xylella fastidiosa gene expression analysis by DNA microarrays.Genetics and molecular biology · 2009Article
- Characterization of DNA hybridization kinetics in a microfluidic flow channel.Sensors and actuators. B, Chemical · 2006Article
- Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts.Nucleic acids research · 2005Article
- Human papillomavirus type 16 E6 promotes retinoblastoma protein phosphorylation and cell cycle progression.Journal of virology · 2004Article
- Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays.BMC genomics · 2004Article
- Identification of novel AP-1 target genes in fibroblasts regulated during cutaneous wound healing.Oncogene · 2004Article
- Negative subtraction hybridization: an efficient method to isolate large numbers of condition-specific cDNAs.BMC genomics · 2004Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the production of DNA microarrays from PCR products, purification of the the DNA fragments prior to spotting is a major expense in cost and time. Also, a considerable amount of material is lost during this process and contamination might occur. Here, a protocol is presented that permits the manufacture of microarrays from unpurified PCR products on aminated surfaces such as glass slides coated with the widely used poly(L-lysine) or aminosilane. The presence of primer molecules in the PCR sample does not increase the non-specific signal upon hybridisation. Overall, signal intensity on arrays made of unpurified PCR products is 94% of the intensity obtained with the respective purified molecules. This slight loss in signal, however, is offset by a reduced variation in the amount of DNA present at the individual spot positions across an array, apart from the considerable savings in time and cost. In addition, a larger number of arrays can be made from one batch of amplification products.
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