ArticleThe Journal of cell biology2002
Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 150 citations in OpenAlex.
- Cell deformability enhances adhesion of ATRA-differentiated HL-60 cells to P-selectin.European journal of medical research · 2025Article
- Review
- Nanoscopic Characterization of Cell Migration under Flow Using Optical and Electron Microscopy.Analytical chemistry · 2023Article
- Old and New Blood Markers in Human Colorectal Cancer.International journal of molecular sciences · 2022Review
- The N-terminal autoinhibitory module of the A1 domain in von Willebrand factor stabilizes the mechanosensor catch bond.RSC chemical biology · 2022Article
- The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.Journal of cell science · 2021Article
- Neutrophils lacking ERM proteins polarize and crawl directionally but have decreased adhesion strength.Blood advances · 2020Article
- Localization of Rolling and Firm-Adhesive Interactions Between Circulating Tumor Cells and the Microvasculature Wall.Cellular and molecular bioengineering · 2020Article
- Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.Cellular and molecular bioengineering · 2018Article
- Computational study of cell adhesion and rolling in flow channel by meshfree method.Computer methods in biomechanics and biomedical engineering · 2017Article
- Surface deformation and shear flow in ligand mediated cell adhesion.Journal of mathematical biology · 2016Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- Dual functions of Rap1 are crucial for T-cell homeostasis and prevention of spontaneous colitis.Nature communications · 2015Article
- Analytical cell adhesion chromatography reveals impaired persistence of metastatic cell rolling adhesion to P-selectin.Journal of cell science · 2015Article
- Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics.Thrombosis research · 2015Article
- Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cytoskeletal regulation of CD44 membrane organization and interactions with E-selectin.The Journal of biological chemistry · 2014Article
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Leukocytes roll on selectins at nearly constant velocities over a wide range of wall shear stresses. Ligand-coupled microspheres roll faster on selectins and detach quickly as wall shear stress is increased. To examine whether the superior performance of leukocytes reflects molecular features of native ligands or cellular properties that favor selectin-mediated rolling, we coupled structurally defined selectin ligands to microspheres or K562 cells and compared their rolling on P-selectin. Microspheres bearing soluble P-selectin glycoprotein ligand (sPSGL)-1 or 2-glycosulfopeptide (GSP)-6, a GSP modeled after the NH2-terminal P-selectin-binding region of PSGL-1, rolled equivalently but unstably on P-selectin. K562 cells displaying randomly coupled 2-GSP-6 also rolled unstably. In contrast, K562 cells bearing randomly coupled sPSGL-1 or 2-GSP-6 targeted to a membrane-distal region of the presumed glycocalyx rolled more like leukocytes: rolling steps were more uniform and shear resistant, and rolling velocities tended to plateau as wall shear stress was increased. K562 cells treated with paraformaldehyde or methyl-beta-cyclodextrin before ligand coupling were less deformable and rolled unstably like microspheres. Cells treated with cytochalasin D were more deformable, further resisted detachment, and rolled slowly despite increases in wall shear stress. Thus, stable, shear-resistant rolling requires cellular properties that optimize selectin-ligand interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.