ArticleBMC molecular biology2002
A comparison of efficacy and toxicity between electroporation and adenoviral gene transfer.
Article in BMC molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Between defence and delivery: the DNA sensing response to gene electrotransfer.Radiology and oncology · 2025Review
- MicroRNAs: Small Molecules with Significant Functions, Particularly in the Context of Viral Hepatitis B and C Infection.Medicina (Kaunas, Lithuania) · 2023Review
- CORP: Gene delivery into murine skeletal muscle using in vivo electroporation.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Review
- Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells.Scientific reports · 2021Article
- Innate signalling molecules as genetic adjuvants do not alter the efficacy of a DNA-based influenza A vaccine.PloS one · 2020Article
- Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations.Nature materials · 2019Article
- The proper strategy to compress and protect plasmid DNA in the Pluronic L64-electropulse system for enhanced intramuscular gene delivery.Regenerative biomaterials · 2019Article
- A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle.International journal of molecular sciences · 2019Review
- Biodegradable nanoneedles for localized delivery of nanoparticles in vivo: exploring the biointerface.ACS nano · 2015Article
- Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.Nature materials · 2015Article
- Myosin light chain 3f attenuates age-induced decline in contractile velocity in MHC type II single muscle fibers.Aging cell · 2012Article
- Physiological and histological changes in skeletal muscle following in vivo gene transfer by electroporation.American journal of physiology. Cell physiology · 2011Article
- Polyvalent nucleic acid nanostructures.Journal of the American Chemical Society · 2011Article
- Plasmid injection and application of electric pulses alter endogenous mRNA and protein expression in B16.F10 mouse melanomas.Cancer gene therapy · 2010Article
- MicroRNA: Biogenesis, Function and Role in Cancer.Current genomics · 2010Article
- Ultrasound-based nonviral gene delivery induces bone formation in vivo.Gene therapy · 2008Article
- Molecular-targeted therapy for Duchenne muscular dystrophy: progress and potential.Molecular diagnosis & therapy · 2008Review
- Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo.Bioelectrochemistry (Amsterdam, Netherlands) · 2007Article
- Gene expression profiles in skeletal muscle after gene electrotransfer.BMC molecular biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundElectroporation of skeletal muscle after injection of naked DNA was shown by others to increase transgene expression. Information regarding tissue damage caused by electroporation is conflicting. It is also not well known how plasmid electroporation compares with transfection by adenoviral vectors. To investigate these questions the most used protocol for muscle electroporation was used, i.e. 8 pulses of 200 V/cm and 20 ms at a frequency of 1 Hz.
resultsIntra-muscular DNA transfer of pLuciferase was increased by 2 logs after electroporation, confirming data described by others. However, the blood levels of the encoded protein were still lower than those obtained after injection of first generation adenoviral vectors. Also, the electroporation procedure, on its own, caused severe muscle damage consisting of rhabdomyolysis and infiltration, whereas the adenoviral vectors caused only a slight infiltration. As damage of targeted tissue may be an advantage in the case of tumour transfection, we also compared the two transfection methods in tumour tissue. In case of poorly permissive tumours, adenoviral vectors cannot transfect more than 2% of the tumour tissue without inducing significant liver damage. In contrast, the electroporation seems to offer a wider therapeutic window since it does not cause any systemic toxicity and still induce's significant transfection.
conclusionsPlasmid electroporation of the muscle induce severe local damage and is of no advantage over adenoviral vectors for obtaining high blood levels of a vector encoded protein. In contrast, electroporation of tumours might be safer than adenoviral gene transfer.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.