ArticleWorld journal of gastroenterology2002
Apoptosis of rat hepatic stellate cells induced by anti-focal adhesion kinase antibody.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Novel monoclonal antibody against integrin α3 shows therapeutic potential for ovarian cancer.Cancer science · 2020Article
- Artesunate may inhibit liver fibrosis via the FAK/Akt/β-catenin pathway in LX-2 cells.BMC pharmacology & toxicology · 2018Article
- Adiponectin as an anti-fibrotic and anti-inflammatory adipokine in the liver.Current pathobiology reports · 2015Article
- Imatinib mesylate improves liver regeneration and attenuates liver fibrogenesis in CCL4-treated mice.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2012Article
- Focal adhesion kinase: a prominent determinant in breast cancer initiation, progression and metastasis.Cancer letters · 2010Review
- Small molecule chloropyramine hydrochloride (C4) targets the binding site of focal adhesion kinase and vascular endothelial growth factor receptor 3 and suppresses breast cancer growth in vivo.Journal of medicinal chemistry · 2009Article
- Activation of focal adhesion kinase enhances the adhesion and invasion of pancreatic cancer cells via extracellular signal-regulated kinase-1/2 signaling pathway activation.Molecular cancer · 2005Article
- Focal adhesion kinase suppresses apoptosis by binding to the death domain of receptor-interacting protein.Molecular and cellular biology · 2004Article
- Antiproliferative and proapoptotic effects of somatostatin on activated hepatic stellate cells.World journal of gastroenterology · 2004Article
- Hepatocellular apoptosis after hepatectomy in obstructive jaundice in rats.World journal of gastroenterology · 2003Article
- Peroxisome proliferator-activated receptor gamma ligands inhibit cell growth and induce apoptosis in human liver cancer BEL-7402 cells.World journal of gastroenterology · 2003Article
- Effects of the tyrosine protein kinase inhibitor genistein on the proliferation, activation of cultured rat hepatic stellate cells.World journal of gastroenterology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo explore the role of focal adhesion kinase (FAK) in the apoptosis in culture-activated rat hepatic stellate cells (HSCs) using a specific anti-FAK antibody.
methodsRat HSCs were prepared from Wistar rats by in situ perfusion of collagenase and pronase and single-step density Nycodenze gradient. Culture-activated HSCs were serum-starved and treated with the anti-FAK antibodies for 24, 48 or 72 h. The apoptosis of HSC was detected by DNA-fragment assay, flow cytometry and caspase-3 activity determination. The expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA was assessed by reverse transcription polymerase chain reaction (RT-PCR).
resultsThe experiment showed that anti-FAK antibodies induced apoptosis of culture-activated rat HSCs. This phenomenon displayed the classical features of apoptotic cell death (DNA fragmentation, cell cycle analysis) after treated with 30 mg.L(-1) FAK antibody for 72 h, and accompanied by a significant increase of caspase-3 activity(1208+/-76) vs (309+/-28) nmol.min(-1).g(-1), t=208.5, P<0.05. Meanwhile, treatment with the FAK antibody in HSCs could markedly decrease the TIMP-1 mRNA expression (0.07+/-0.01 vs 0.38+/-0.03, t=2.72, P<0.05).
conclusionFAK plays an important role in the survival of HSCs and the specific anti-FAK antibody could induce the apoptosis in rat HSCs.
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