ArticleWorld journal of gastroenterology2002
Effect of cholecystokinin octapeptide on tumor necrosis factor alpha transcription and nuclear factor-kappaB activity induced by lipopolysaccharide in rat pulmonary interstitial macrophages.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of probiotic on intestinal mucosa of patients with ulcerative colitis.World journal of gastroenterology · 2004Trial
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- Review
- Heterogeneity and plasticity of porcine alveolar macrophage and pulmonary interstitial macrophage isolated from healthy pigsBiology open · 2019Article
- Expression and Regulation of Cholecystokinin Receptor in the Chicken's Immune Organs and Cells.Journal of clinical & cellular immunology · 2016Article
- Cholecystokinin protects rats against sepsis induced by Staphylococcus aureus.Medical microbiology and immunology · 2014Article
- Cholecystokinin inhibits inducible nitric oxide synthase expression by lipopolysaccharide-stimulated peritoneal macrophages.Mediators of inflammation · 2014Article
- Protective effect of ginsenoside Rb1 against lung injury induced by intestinal ischemia-reperfusion in rats.Molecules (Basel, Switzerland) · 2013Article
- Ginsenoside Rb1 attenuates intestinal ischemia-reperfusion- induced liver injury by inhibiting NF-kappaB activation.Experimental & molecular medicine · 2008Article
- Enteroendocrine cells express functional Toll-like receptors.American journal of physiology. Gastrointestinal and liver physiology · 2007Article
- Expression of NF-kappaB and human telomerase reverse transcriptase in gastric cancer and precancerous lesions.World journal of gastroenterology · 2004Article
- Hepatocellular apoptosis after hepatectomy in obstructive jaundice in rats.World journal of gastroenterology · 2003Article
- Expression and cell-specific localization of cholecystokinin receptors in rat lung.World journal of gastroenterology · 2003Article
- The mRNA expression patterns of tumor necrosis factor-alpha and TNFR-I in some vital organs after thermal injury.World journal of gastroenterology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo elucidate the anti-inflammatory mechanism of an intestinal neuropeptide, sulfated cholecystokinin octapeptide (sCCK-8), the effects of sCCK-8 on lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-alpha) mRNA expression and NF-kappaB activity in pulmonary interstitial macrophages (PIMs) were studied.
methodsPIMs from rat were stimulated with LPS (1 mg.L(-1)) in the presence or absence of sCCK-8 (10(-8)-10(-6)mol.L(-1)) or/and CCK receptor antagonist proglumide (2 mg.L(-1)). The expression of TNF-alpha mRNA was assayed by reverse transcription polymerase chain reaction (RT-PCR) at 3h of the stimulation, and nuclear factor-kappaB (NF-kappaB) binding activity was analyzed by electrophoretic mobility shift assay (EMSA) at 1 h of stimulation. The IkappaBalpha protein level in the cytoplasma at 30 min of the stimulation was detected by Western blot.
resultssCCK-8, at concentrations from 10(-8) mol.L(-1) to 10(-6) mol.L(-1) obviously inhibited LPS-induced TNF-alpha mRNA expression and NF-kappaB binding activity in a dose-dependent manner, P<0.05, P<0.01. Stimulation PIMs with LPS resulted in a reduction of IkappaBalpha protein level, P<0.01, which was elevated by sCCK-8, P<0.05. The effects of sCCK-8 on NF-kappaB activity and IkappaB protein level were attenuated by CCK receptor antagonist proglumide, P<0.01.
conclusionsCCK-8 inhibits LPS-induced TNF-alpha mRNA expression by regulating NF-kappaB activity in rat PIMs, which is mediated through CCK receptors and inhibiting IkappaB-alpha degradation. This represents one of the anti-inflammatory mechanisms of sCCK-8.
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